姜黄素对APP/PS1双转基因鼠海马ABCA1、apoA1的表达和血清TC、HDL含量的影响

Effects of curcumin on serum total cholesterol and high-density lipoprotein contents and expression of ABCA1 and apoA1 in CA1 region of hippocampus in APP/PS1 double transgenic mice

摘要:

目的：探讨姜黄素对APP/PS1双转基因鼠海马组织中三磷酸腺苷结合盒转运子A1（ATP binding cassette transport pro－tein A1，ABCA1）和载脂蛋白A1（apolipoprotein A1，apoA1）的表达及血清中总胆固醇（total cholesterol，TC）和高密度脂蛋白（high-density lipoprotein，HDL）含量的影响。方法：用APP/PS1双转基因鼠建立阿尔茨海默病（Alzheimer’s disease，AD）模型，不同浓度姜黄素饲料喂养6个月。免疫组化SP法检测转基因鼠的海马组织CA1区ABCA1和apoA1的表达变化。胆固醇酶法比色法检测血清中TC和HDL的含量。结果：经过不同浓度姜黄素饲喂转基因鼠后，其海马组织CA1区ABCA1和apoA1的表达增加（P=0.005和0.003；P=0.025和0.001），且血清中HDL的含量也随之增加，TC的含量逐渐减少，其差异均有统计学意义（P=0.041和0.010；P=0.046和0.002）。结论：ABCA1在AD的发生发展中起着重要的作用，姜黄素可能是通过增加ABCA1表达和升高apoA1和HDL含量降低胆固醇水平。

Abstract:

Objective：To investigate the effect of curcumin on serum total cholesterol（TC） and high-density lipoprotein（HDL） and expression of ATP binding cassette transport protein A1（ABCA1） and apolipoprotein A1（apoA1） in CA1 region of hippocampus in APP/PS1 double transgenic mice. Methods：Alzheimer’s disease（AD） model was established with APP/PS1 double transgenic mice，which were fed for 6 months with different concentrations of curcumin diet. Immunohistochemistry（SP） was applied to evaluate the expressive change of ABCA1 and apoA1 in CA1 region of hippocampus of transgenic mice. Colorimetric method was applied to detect the content of TC and HDL in blood serum of transgenic mice. Results：After feeding transgenic mice with curcumin diet，expression of ABCA1 and apoA1 in CA1 region of hippocampus was increased in concentration dependent manner（P=0.005，0.003；P=0.025，0.001）. Content of HDL in blood serum was increased too while TC was decreased with significant differences between groups（P=0.041，0.010；P=0.046，0.002）. Conclusions：ABCA1 may play an important role in the generation or development of AD. Curcumin may lower the content of cholesterol by raising expression of ABCA1 and content of apoA1 and HDL.