结直肠癌组织中miRNA的差异表达研究

Differential expression of microRNAs in human colorectal cancer tissues

摘要:

目的：寻找并鉴定在结直肠癌及癌旁正常组织中差异表达的miRNA，为进一步阐明其在结直肠癌发生、发展机制中的作用奠定实验基础。方法：收集10例新鲜的结直肠癌及癌旁正常组织，抽提总RNA，利用miRNA芯片技术寻找结直肠癌及癌旁正常组织中差异表达的miRNA，并通过real-time PCR技术进行验证。结果：与结直肠癌旁正常组织相比，结直肠癌组织中上调2.3倍以上的miRNA有32个，下调2倍以上的miRNA有10个；real-time PCR结果与miRNA芯片结果较吻合。结论：筛选得到结直肠癌miRNA差异表达谱，其可能与结直肠癌的发生、发展相关。

Abstract:

Objective：To identify the differentially expressed microRNAs（miRNAs） in human colorectal cancer tissues and to make further study on the function of miRNA in pathogenesis and development mechanism of colorectal cancer. Methods：Total RNA was extracted from 10 samples fresh colorectal cancer tissues and normal adjacent tissues and the differentially expressed miRNAs were detected by miRNA array. Real-time PCR was applied to verify the reliability of miRNA array results. Results：miRNA array results showed that 32 miRNAs were up-regulated more than 2.3 folds and 10 miRNAs were down-regulated more than 2 folds in colorectal cancer tissues compared with those in normal adjacent colorectal tissues. And the real-time PCR results were in accordance with the miRNA array results. Conclusion：The miRNA differential expression profile of colorectal cancer is obtained，which might relate with the pathogenesis and development of colorectal cancer.