ERRα mRNA及蛋白在乳腺癌中的表达及其临床意义

mRNA and protein expression of estrogen receptor-related receptor α in breast cancer and its clinical significance

摘要:

目的：研究雌激素受体相关受体α（estrogen receptor-related receptor α，ERRα）mRNA和蛋白在乳腺癌组织及乳腺癌旁组织的表达及意义，进一步探讨ERRα与乳腺癌临床病理指标[雌激素受体（estrogen receptor，ER）、孕激素受体（progesterone re－ceptor，PR）、人表皮生长因子受体-2（human epidemic growth factor receptor-2，Her-2）、P53]，以及与乳腺癌分级、分期的相关性。方法：收集乳腺癌及对应乳腺癌旁组织标本87例，分别采用免疫组织化学染色，RT-PCR，Western blot检测ERRα mRNA和蛋白的表达情况，进一步分析ERRα的表达与乳腺癌临床病理特征的关系。结果：本研究中ERRα mRNA和蛋白在乳腺癌组织中的表达均高于在乳腺癌旁组织中的表达（P＜0.05），且ERRα的表达与乳腺癌病理指标Her-2、ER以及肿瘤的病理分级，肿瘤大小，淋巴结转移相关，而与患者的年龄、PR、P53等无明显相关。结论：ERRα在乳腺癌中的高表达，可能作为乳腺癌一个预后不良的新的分子标记物，为乳腺癌的诊治提供新的思路。

Abstract:

Objective：To detect the expression and significance of estrogen receptor-related receptor（ERR） α mRNA and protein in breast cancer and breast para-carcinoma tissues and to further discuss the correlation between ERR α and breast cancer clinical pathological index（（estrogen receptor（ER），progestin receptor（PR），human epidermal growth factor receptor-2（Her-2），P53） as well as the grade and classification of breast cancer. Methods：Expression of ERRα in 87 breast cancer tissues and the corresponding breast para-carcinoma tissues were detected by immunohistochemistry and reverse transcription PCR（RT-PCR），Western blot respec－tively and their relationship with the clinical pathological characteristics was analyzed. Results：Expression of ERR α mRNA and pro－tein in breast cancer tissues was higher than that in breast para-carcinoma tissues（P<0.05）. Expression of ERR α was correlated with ER，Her-2，degrees of differentiation，tumor size and metastasis of the lymph nodes but was not correlated with patient’s age，PR and P53. Conclusions：High expression of ERR α may serve as a biomarker of poor prognosis in breast cancer and it may provide new thought for the diagnosis and treatment of breast cancer.