血红素加氧酶1.2在髓母细胞瘤中的表达及意义

Expression and significance of HO-1 and HO-2 in medulloblastoma

摘要:

目的：探讨血红素加氧酶（heme oxygenase，HO）-1和HO-2在髓母细胞瘤（medulloblastoma，MB）中的表达及意义。方法：采用SP免疫组织化学法检测41例MB组织和23例对照脑组织标本中HO-1和HO-2的表达，分析HO-1和HO-2的表达相关性及其与MB的临床特征（性别、年龄、病理类型和肿瘤部位）之间的关系。结果：在41例MB中，HO-1和HO-2的阳性表达率分别为78.0%（32/41）和73.2%（30/41），高于对照脑组织（P=0.002、0.001），且二者在MB中的表达呈正相关（rs=0.477，P=0.009）；HO-1和HO-2在MB中的表达与患者的性别、年龄和肿瘤部位等临床特征均无相关性，差异无统计学意义（HO-1：?字2=0.118、0.000、2.903，P=0.732、1.000、0.424；HO-2：?字2=2.737、1.191、1.169，P=0.098、0.662、0.945），但HO-1和HO-2在不同组织病理学类型的MB中的表达具有统计学差异（HO-1：?字2=12.570，P=0.002；HO-2：?字2=6.677，P=0.044）。结论：HO-1和HO-2的高表达在MB的生长中发挥重要作用。HO-1和HO-2的表达与MB的组织病理学分型密切相关，提示HO-1和HO-2可作为评估患者病情和预后的新指标及治疗新靶点。

Abstract:

Objective：To investigate the expression and clinical values of heme oxygenase（HO）-1 and HO-2 in medulloblastoma. Methods：Expression of HO-1 and HO-2 in 41 cases of medulloblastoma and 23 cases of control brain tissues was determined by im－munohistochemical assay with SP staining. Relationship between HO-1 and HO-2 expression as well as relationship between HO-1 and HO-2 expression and clinical features（sex，age，histopathology and tumor location） were analyzed with corresponding statistical meth－ods. Results：Expression rates of HO-1 and HO-2 in 41 cases of medulloblastoma were 78.0%（32/41） and 73.2%（30/41） respective－ly，significantly higher than those in control brain tissue（P=0.002，0.001）. Expression of HO-1 was positively related with expression of HO-2（rs=0.477，P=0.009）. There was no difference in gender，age and tumor location between negative and positive of HO-1 and HO-2 groups（HO-1：?字2=0.118，0.000，2.903；P=0.732，1.000，0.424；HO-2：?字2=2.737，1.191，1.169，P=0.098，0.662，0.945）. However，expression of HO-1 and HO-2 showed significant difference in medulloblastoma with different histopathology types（HO-1： ?字2=12.570，P=0.002；HO-2：?字2=6.677，P=0.044）. Conclusions：High expression of HO-1 and HO-2 play an important role in medulloblastoma growth. Futhermore，expression of HO-1 and HO-2 is significantly associated with histopathology of medulloblastoma，indicating that HO-1 and HO-2 may be used as new marker for patient’s state，prognosis and therapeutic target.