鼻咽癌癌变前后ADAM23、MIMT1、FAM150B、GRIN2A、LRRC4甲基化研究

Study on methylation of ADAM23，MIMT1，FAM150B，GRIN2A，LRRC4 before and after nasopharyngeal carcinoma canceration

摘要:

目的：研究鼻咽癌变前后ADAM23、MIMT1、FAM150B、GRIN2A、LRRC4甲基化特征。方法：12例鼻咽癌10例鼻咽炎作为研究对象，抽提鼻咽组织DNA，以特异识别5-甲基胞嘧啶的抗体免疫沉淀DNA，实时荧光定量PCR分别检测5个基因含量，并设置不用5-甲基胞嘧啶抗体（非MeDIP-qPCR实验）及使用非特异性抗体IgG进行免疫沉淀作为双重对照。结果：无论鼻咽癌还是鼻咽炎患者，5个基因在使用非特异性抗体IgG实验时均未检验到甲基化，非MeDIP-qPCR实验显示5个基因在鼻咽癌及鼻咽炎荧光定量值无明显差异（P >0.05）；MeDIP-qPCR实验显示，鼻咽癌5个基因均呈现明显的甲基化，ADAM23、MIMT1、FAM150B、GRIN2A、LRRC45在鼻咽癌校正值分别为：3.39±2.28、6.03±3.93、3.68±1.81、7.12±3.17、3.10±1.94，在鼻咽炎分别为0.00±0.00、0.01±0.01、0.25±0.49、0.20±0.20、0.00±0.00（均P<0.01）。结论：ADAM23、MIMT1、FAM150B、GRIN2A、LRRC4 5个基因甲基化为鼻咽癌变的重要特征。

Abstract:

Objective：to study ADAM23，MIMT1，FAM150B，GRIN2A，LRRC4 DNA methylation feature while nasopharyngeal epithe－lium canceration. Methods：Totally 12 patients with nasopharyngeal carcinoma and 10 patients with nasopharyngitis were chose as the research objects. Nasopharyngeal tissue DNA was extracted and was used to specifically identify 5-methyl cytosine antibody immune precipitation DNA. Real-time fluorescent quantitative PCR was used to detect the contents of five genes respectively. Non 5-methyl cytosine antibody（non MeDIP-qPCR experiment） and nonspecific antibody IgG immune precipitation were set up as dual controls. Results：Regardless of the patients with nasopharyngeal carcinoma or nasopharyngitis，methylation was not detected in five genes when using nonspecific antibody IgG experiment. Non MeDIP-qPCR experiment showed that there was no difference in fluorescence quan－titative value of five genes in nasopharyngeal carcinoma and nasopharyngitis（P >0.05）. MeDIP-qPCR experiment showed that all five genes in nasopharyngeal carcinoma presented significant methylation. Corrected values of real-time fluorescence quantification of ADAM23，MIMT1，FAM150B，GRIN2A，LRRC45 were 3.39±2.28，6.03±3.93，3.68±1.81，7.12±3.17，3.10±1.94 in nasopharyn－geal carcinoma and were 0.00±0.00，0.01±0.01，0.25±0.49，0.20±0.20，0.00±0.00 in nasopharyngitis（all P < 0.01）. Conclusion：ADAM23，MIMT1，FAM150B，GRIN2A，LRRC4 gene methylation are important characteristics of nasopharyngeal carcinoma.