CCCTC-结合因子在子宫内膜癌中的表达及诊断意义

Expression and diagnostic significance of CCCTC-binding factor in endometrial carcinoma

摘要:

目的：探讨CCCTC-结合因子（CCCTC-binding factor，CTCF）在子宫内膜癌中的表达及临床诊断意义。方法：随机选取30例正常子宫内膜标本（其中15例增殖期内膜组织，15例分泌期内膜组织）为对照组，45例子宫内膜癌患者的子宫内膜标本为实验组。采用Western blot 法检测CTCF蛋白和人端粒酶逆转录酶（human telomerase reverse transcriptase，hTERT）在各组中的表达水平以及相关性；半定量RT-PCR法检测各组中CTCF基因mRNA及hTERT基因mRNA转录水平。结果：CTCF和hTERT在各组中均有表达。实验组CTCF蛋白和hTERT基因蛋白阳性表达率及相对表达量与对照组（分别88.89% vs. 60% ?字2=8.57，P=0.005；80% vs. 26.67%， ?字2=21.11，P=0.000；t=50.039，P=0.000；t=10.662，P=0.000）间存在差异；实验组CTCF基因mRNA和hTERT基因mRNA相对表达量与对照组（分别t=67.448，P=0.000；t=65.908，P=0.000）间同样具有统计学差异性。CTCF的表达与子宫内膜癌的病理分级和临床手术分期均有关（分别 ?字2=8.613，P=0.007； ?字2=11.739，P=0.001），与病理类型无关（?字2=4.906，P=0.086）；hTERT的表达与子宫内膜癌病理分级、病理类型、临床分期均无关（分别 ?字2=0.352，P=0.258； ?字2=1.568，P=0.457； ?字2=0.000，P=1.000）。子宫内膜癌中CTCF和hTERT的异常表达存在相关性（?字2=5.625，P=0.018），并呈正相关（r=0.354，P=0.017）。结论：CTCF与端粒酶hTERT在正常子宫内膜组织与子宫内膜癌中表达具有差异性，不同临床参数下CTCF与hTERT表达情况各异，CTCF和hTERT在预测与临床参数关系密切的子宫内膜癌复发过程中起重要作用。

Abstract:

Objective：To investigate the expression and diagnostic significance of CCCTC-binding factor（CTCF） in the endometrium. Methods：Normal endometrium samples collected from 30 healthy women were served as control group（15 samples at proliferation phase and 15 samples at secretion phase） However，endometrium samples collected from 45 patients with endometrial carcinoma were served as test group. Expression level and correlation of CTCF protein and telomerase gene human telomerase reverse transcriptase（hTERT） protein in two groups were determined by Western blot，while the transcription level of CTCF mRNA and telomerase gene hTERT mRNA was detected by semi-quantitative PCR（RT-PCR）. Results：CTCF and hTERT were both expressed in two groups. Positive expression percentage and relative expression quantity for CTCF protein and telomerase gene hTERT protein in test group were both different from those in control group（88.89% vs. 60%，?字2=8.57，P=0.005；80% vs. 26.67%，?字2=21.11，P=0.000；t=50.039，P=0.000；t=10.662，P=0.000）. There were differences in mRNA expression of CTCF and hTERT gene between test group and control group（t=67.448，P=0.000；t=65.908，P=0.000）. Expression of CTCF was associated with endometrial carcinoma histological stages and clinical stages（?字2=8.613，P=0.007； ?字2=11.739，P=0.001），while not with histological subtypes（?字2=4.906，P=0.086）. Meanwhile，expres－sion of hTERT has nothing to do with histological stages，histological subtypes and clinical stage（?字2=0.352，P=0.258；?字2=1.568，P=0.457； ?字2=0.000，P=1.000）. Expression of CTCF was positively correlated with hTERT in endometrial carcinoma（?字2=5.625，P=0.018；r=0.354，P=0.017）. Conclusions：CTCF and telomerase hTERT are differently expressed in endometrial carcinoma and normal en－dometrial tissues. Expression of CTCF and telomerase hTERT is also different under different clinical parameters. CTCF and hTERT might play an important role in forecasting endometrial carcinoma recurrence which has close relationship with clinical parameters.