慢性脑缺血模型小鼠视网膜内血管内皮生长因子和胶质纤维酸性蛋白的表达变化

Changes in the expression of vascular endothelial growth factor and glial fibrillary acidic protein in the retina of mice with chronic brain ischemia

摘要:

目的：探讨血管内皮生长因子（vascular endothelial growth factor，VEGF）和胶质纤维酸性蛋白（glial fibrillary acidic pro－tein，GFAP）在慢性脑缺血模型小鼠视网膜内的表达规律及两者的关系。方法：采用C57小鼠建立慢性脑缺血模型，结合免疫组化方法，观察缺血不同时间段小鼠视网膜中 VEGF、GFAP在时间和空间上的表达变化。结果：在假手术组中 VEGF、GFAP呈弱表达或不表达；在手术组中，缺血120 d时 VEGF、GFAP表达增加（P=0.002，P=0.006），随缺血时间的延长，缺血180 d时 VEGF、GFAP表达强度及空间分布范围呈进行性增加（P=0.050，P=0.000）。结论：VEGF、GFAP在慢性脑缺血模型小鼠视网膜中表达进行性上调，提示两者参与了视网膜缺血损伤的病理改变过程，并在视网膜缺血损伤机制中起着重要作用。

Abstract:

Objective：To examine the expression of vascular endothelial growth factor（VEGF） and glial fibrillary acidic protein（GFAP） in the retina of mice with chronic brain ischemia as well as to analyze the relationship between VEGF and GFAP under retinal is－chemic injury. Methods：C57 mice were used to establish chronic brain ischemia model. Immunohistochemical staining was performed to observe the distribution and expression of VEGF and GFAP in the mice retina at different ischemic time periods. Results：VEGF and GFAP were unexpressed or weakly expressed in sham operated group. In the operated group，expression of VEGF and GFAP was in－creased at 120 d after ischemia and was significantly increased in intensity and scope at 180 d after ischemia. Conclusions：Expression of VEGF and GFAP are increased in the retina of mice with chronic brain ischemia，which indicates VEGF and GFAP may be involved in the pathogenesis of retinal ischemic injury process.