依达拉奉对蛛网膜下腔出血后急性脑血管痉挛大鼠的影响

Effects of edaravone on acute cerebral vascular spasm after subarachnoid hemorrhage in rats Ma Haihua1，Pan Juan2，Li Da2

摘要:

目的：探讨依达拉奉（edaravone，E）对蛛网膜下腔出血（subarachnoid hemorrhage，SAH）大鼠急性脑血管痉挛（cerebral vasospasm，CVS）的保护作用及对血中S100B、内皮素-1（endothelin-1，ET-1）表达水平的影响。方法： 60只Wistar大鼠随机分为对照组（C组）、生理盐水组（N组）、单纯SAH组（SAH组）和 SAH +依达拉奉组（SAH + E组）。应用枕大池二次注入自身动脉血法制作大鼠 SAH后急性 CVS动物模型，C组于术前，其余3组分别于术后 6、24、72 h采用显微图像分析仪测量各组基底动脉直径，并ELISA法测定各组血清S100B、ET-1水平。结果：术后同一时间点基底动脉直径由小到大依次为：SAH组、SAH+E组、N组、C组，除N、C组之间无统计学差异外，其余两两比较均有统计学意义，析因分析提示仅不同处理方法对结果有影响；基底动脉72 h内持续痉挛，时间无影响；术后同一时间点血清S100B 、ET-1水平由高到低依次为：SAH组、SAH+E组、N组、C组，除N、C组之间无统计学差异外，其余两两比较均有统计学意义，析因分析提示仅不同处理方法对结果有影响；表达量72 h内持续增高，差异有统计学意义（P<0.05）。结论：血清S100B水平和ET-1水平的变化与SAH后急性CVS有关。依达拉奉的应用能够使血清S100B、ET-1水平恢复正常从而起到改善SAH后急性CVS的作用。

Abstract:

Objective：To explore the protection of edaravone on acute cerebral vasospasm（CVS） after subarachnoid hemorrhage（SAH） in rats and effects of edaravone on expressions of serum S100B and endothelin-1（ET-1）. Methods：Totally 60 Wistar rats were randomly assigned to four groups：control group（group C），normal saline group（group N），simple group of SAH（group SAH） and group of SAH+ edaravone（group SAH+E）. CVS model after SAH was made by injecting rats’ own blood into cisterna twice. Microscopic im－age analysis was used to measure the basal artery diameter and ELISA was employed to determine S100B and ET-1serum levels pre－operatively in group C and at 6，24，72 h postoperatively in others groups. Results：Postoperative basilar artery diameters at the same time were in the following order（from small to large）：group SAH，group SAH + E，group N and group C. There was no statistically significant difference between groups N and C and the differences were statistically significant between the other groups. Factorial anal－ysis suggested that different treatments exerted different effects on the results. Basilar artery spasms sustained for 72 h. At the same time after operation，serum S100B and ET-1 levels were in the following order（from high to low）：group SAH，group SAH+E，group N and group C. There were statistically significant differences between groups but there was no difference between groups N and C. Fac－torial analysis suggested that different treatments exerted different effects on the results. Serum S100B and ET-1 increased consis－tently within 72 h，with statistically significant differences（P<0.05）. Conclusions：Serum S100B and ET-1 levels related with acute CVS after SAH. Edaravone can make serum S100B and an ET-1 level back to normal and thus plays a controlling role in improving acute CVS after SAH.