具备不同拓扑结构的聚甲基丙烯酸甲酯静电纺丝纳米纤维细胞支架的构建及鉴定

Construction and evaluation of electrospun polymethylmethacrylate nanofibers with different topographic features as biological cell scaffold

摘要:

目的：构建具有不同拓扑结构的聚甲基丙烯酸甲酯（polymethylmethacrylate，PMMA）电纺纳米纤维，并探讨其作为植入性细胞支架的可行性。方法：采用静电纺丝技术构建具有随机或有序拓扑结构的PMMA电纺纳米纤维，利用PMMA薄膜作为对照，以其为基底培养PC12细胞，探讨PC12细胞在纤维支架上的神经突生长能力以及神经突对局部拓扑环境的反应。结果：成功制作表观良好的随机或有序PMMA电纺纤维，在2种拓扑结构的纤维系统上，纤维均在纳米层级，直径无统计学差异（t=1.079，P=0.474）；PC12细胞能够在其上顺利贴壁、生长并分化，并能对电纺纤维的拓扑结构作出准确反应，在有序纤维系统上分化的神经突能够和纤维方向保持高度一致，且与在PMMA薄膜或随机电纺纤维上的细胞相比能够生成更长的神经突，差异有统计学意义（q=35.36，P=0.006；q=29.23，P=0.009）。结论：有序PMMA电纺纳米纤维具有作为神经损伤后植入性细胞支架的潜力。

Abstract:

Objective：To produce polymethylmethacrylate（PMMA） electrospun nanofibers with different topographic features and to test their potential as scaffold after neural injury. Methods：Electrospining was used to produce nanofibers with random and aligned topographic features，PC12 cells was cultured on nanofibers and PMMA film was served as control to reveal the respective neurite growth manner on different substrate. Results：Both the random and aligned PMMA electrospun nanofibers had fine surface character and there was no difference in fiber diameter detected（t=1.079，P=0.474）. PC12 cell could successfully adhere，grow and differentiate on each kind of substrate，and on aligned nanofibers the neurite which were also aligned as the orientation of the fibers were significantly longer than those cells cultured on random fibers and film（q=35.36，P=0.006；q=29.23，P=0.009）. Conclusion：Aligned PMMA electrospun nanofibers has the potentiality as scaffold after neural injury.