超高效液相色谱法同时测定抗病毒口服液中3种核苷的含量

Determination of the content of three nucleosides in Kangbingdu oral liquid by ultra performance liquid chromatography

摘要:

目的：建立以超高效液相色谱法（ultra performance liquid chromatography，UPLC）测定抗病毒口服液中尿苷、鸟苷和腺苷含量的方法。方法：色谱柱为ACQUITY UPLC BEH C18（1.7 μm，2.1 mm×50 mm），流动相为水-甲醇不同比例梯度洗脱，柱温30 ℃，流速0.5 ml/min，检测波长为254 nm。结果：尿苷在2.033～20.33 μg/ml浓度范围内线性关系良好，回归方程为：Y=80 664X-1 046（r=0.999 9）；平均回收率为98.56%，相对标准偏差（relative standard deviation，RSD）=1.21%，鸟苷在1.248～12.48 μg/ml浓度范围内线性关系良好，回归方程为：Y=55 261X-742（r=0.999 9）；平均回收率为98.77%，RSD=1.09%，腺苷在1.254～12.54 μg/ml浓度范围内线性关系良好，回归方程为：Y=52 155X-480（r=0.999 9）；平均回收率为98.88%，RSD=1.22%。结论：该方法准确、快速、可靠，能有效地测定抗病毒口服液中3种核苷的含量。

Abstract:

Objective：To establish an ultra performance liquid chromatography（UPLC） method for detecting content of uridine，guanosine and adenosine in Kangbingdu oral liquid. Methods：ACQUITY UPLC BEH C１8（1.7 μm，2.1 mm×50 mm） was used and the mobile phase was methanol and water by gradient elution mode；column temperature was 30 ℃，at a flow rate of 0.5 ml/min and detection wavelength of 254 nm. Results：The linear range of uridine was 2.033-20.33 μg/ml and the regression equation was as follows：Y=80 664X-1 046（r=0.999 9）. The linear range of guanosine was 1.248-12.48 μg/ml and the regression equation was as follows：Y=55 261X-742（r=0.999 9）. The linear range of adenosine was 1.254-12.54 μg/ml and the regression equation was as follows：Y=52 155X -480（r=0.999 9）. The average recovery rates were 98.56％（RSD=1.21％），98.77％（RSD=1.09％） and 98.88％（RSD=1.22％） respectively. Conclusions：The method is accurate，rapid and reliable and can be used to determine the nucleoside compounds in Kangbingdu oral liquid.