3种药物对饲服TCDD的胎鼠腭突超微结构的影响

Effects of three drugs on mouse embryonic palate ultrastructure after feeding TCDD

摘要:

目的：观察叶酸、白藜芦醇和α-萘黄酮拮抗四氯二苯二噁英（2，3，7，8-tetrachlorodibenzo-p-dioxin，TCDD）诱导胎鼠腭裂发生的效应，及对饲服TCDD的胎鼠腭突表面超微结构的影响。方法：妊娠第10天（gestation day 10，GD10）孕鼠随机分组，每组8只。TCDD组以TCDD 28 μg/kg灌胃，对照组以玉米油0.1 ml灌胃，叶酸组以叶酸10 mg/kg + TCDD 28 μg/kg灌胃、白藜芦醇组以白藜芦醇50 mg/kg+TCDD 28 μg/kg灌胃、α-萘黄酮组以α-萘黄酮5 mg/kg+TCDD 28 μg/kg灌胃，于GD17.5在解剖显微镜下观察胎鼠腭裂的发生率。另取15只孕鼠随机分组，每组3只，处理方法同前，分别于GD13.5、14.5、15.5剪取胎鼠头部，扫描电镜观察腭突表面的超微结构。结果：TCDD组胎鼠腭裂发生率为92.86%，对照组未见胎鼠腭裂形成。叶酸组、白藜芦醇组和α-萘黄酮组胎鼠腭裂发生率分别为73.08%、74.51%、65.52%，与TCDD组相比，差异有统计学意义（P<0.05）。TCDD组腭中嵴上皮细胞表面逐渐破裂、皱缩，伪足结构逐渐减少、消失，最终形成腭裂；对照组、叶酸组、白藜芦醇组和α-萘黄酮组腭中嵴上皮细胞表面光滑整齐、饱满膨胀，表面丝状伪足逐渐增多、延长，一直持续到腭突融合结束。结论：叶酸、白藜芦醇和α-萘黄酮均能恢复小鼠腭中嵴上皮细胞表面的超微结构，从而拮抗TCDD的致腭裂效应。

Abstract:

Objective：To observe the incidence and ultrastructure of folic acid，resveratrol and α-naphthoflavone antagonize 2，3，7，8-tetrachlorodibenzo-p-dioxin（TCDD）-induced fetal mice cleft palate. Methods：On gestation day 10（GD 10），40 pregnant mice were randomly assigned to 5 groups. TCDD group（TCDD 28 μg/kg），control group（corn oil 0.1 ml/kg），folic acid group（folic acid 10 mg/kg+TCDD 28 μg/kg），resveratrol group（resveratrol 50 mg/kg+TCDD 28 μg/kg），α-naphthoflavone group（α-naphthoflavone 5 mg/kg+TCDD 28μg/kg） were gavaged. Incidences of cleft palate of each group were observed on GD17.5. Another 15 pregnant mice were randomly divided into five groups，the same as above. The head coronal sections of the fetal mice were prepared on GD 13.5，14.5，15.5，observed by scanning electron microscopy（SEM）. Results：Incidences of cleft palate were 92.86% in TCDD group，0% in control group，73.08% in folic acid group，74.51% in resveratrol group，65.52% in a-naphthoflavone group. There were significant differ－ences of cleft palate incidence in folic acid，resveratrol and α-naphthoflavone groups compared with that in TCDD group（P<0.05）. The embryonic palatal cells in TCDD group gradually ruptured and shrinkaged and filopodia disapperaed；the filopodia in the surface of the smooth cells gradually increased，extended and continued until the end of the palatal fusion in contral，folic acid，resveratrol，α-naphthoflavone groups. Conclusions：Folic acid，resveratrol and α-naphthoflavone can restore the ultrastructure of mice embryonic palatal cells and antagonize TCDD-induced cleft palate.