当归多糖对急性辐射损伤小鼠骨髓基质细胞及血管内皮生长因子的影响

Effects of angelica polysaccharides on bone marrow vascular endothelial growth factor in acute radiation injured mice

摘要:

目的：研究当归多糖（angelica polysaccharides，APS）对辐射损伤小鼠骨髓基质细胞（bone marrow stromal cell，BMSC）及血管内皮生长因子（vascular endothelial growth factor，VEGF）的保护作用。方法：直线加速器一次性全身均匀X射线（4.0 Gy）照射BALB/c小鼠，照射后腹腔注射不同剂量的APS或生理盐水，并于照射后第7、14、21天处死小鼠，取外周血进行常规检查；观察BMSC贴壁情况、细胞80%贴壁时间；流式细胞仪检测细胞周期和凋亡率；RT-PCR法检测VEGF mRNA的表达水平。结果：与正常组相比，辐射第7、14、21天外周血白细胞及血小板计数明显减少；BMSC贴壁细胞数少且折光性差，达80%贴壁时间明显延长；G0/G1期细胞比例及细胞凋亡率升高；VEGF mRNA的表达降低。辐射APS高剂量组和辐射APS低剂量组均能增加外周血各指标计数，BMSC贴壁细胞数增多且折光性变好，达80%贴壁时间缩短，G0/G1期细胞比例及细胞凋亡率均显著降低，VEGF mRNA的表达显著增加。结论：APS可能通过增强VEGF的表达，提高BMSC的增殖能力，减少其凋亡，从而加速机体造血功能的恢复。

Abstract:

Objective：To research the protection of angelica polysaccharides（APS） on bone marrow stromal cell（ＢＭＳＣ） and vascular endothelial growth factor（ＶＥＧＦ） in radiation injured mice. Methods：BALB/c mice was irradiated by linear accelerator with one-time X ray（4.0 Gy）. Then mice were given different dosages of APS or normal saline by intraperitoneal injection. These mice were killed and the peripheral blood cells were achieved to do routine test at the 7th，14th，21st d after the injection. Adherent situation of BMSC was observed and time of 80% adherent BMSC was recorded. Cell cycle and apoptosis ratio were detected by flow cytometry. VEGF mRNA expression of BMSC was detected by RT-PCR assay. Results：Compared with those in normal group，numbers of peripheral leukocytes and platelet were decreased significantly，adherent cells of BMSC were fewer and refractivity was poorer，time of 80% ad－herent BMSC was extended significantly，percentage of G0/G1 phase and apoptosis rate were increased significantly and VEGF mRNA expression was decreased significantly in radiation group at the 7th，14th，21st d after the radiation. Conclusions：APS may promote the recovery of hematopoietic of mice bone marrow probably by enhancing expression of VEGF mRNA，then increasing proliferation ability of BMSC and reducing its apoptosis.