转录因子激活蛋白-1在Buerger’s病中的表达与意义

Expression and significance of transcription factor activator protein-1 in Buerger’s disease

摘要:

目的：观察转录因子激活蛋白-1（activator protein-1，AP-1）在Buerger’s病患者腰交感神经元内的表达。方法：收集2011年1月至2012年3月行腰交感神经节切除术的Buerger’s病男性患者腰交感神经节标本，Buerger’s病患者组：23例，对照组：12例。应用免疫组化、RT-PCR、Western blot技术测定对照组腰交感神经节神经元AP-1的表达水平并比较其表达差异。结果：Buerger’s病患者腰交感神经节神经元中AP-1的基因表达（P=0.045 9）、蛋白表达（P=0.028 3），高于对照组。结论：AP-1在调节Buerger’s病细胞增殖和血管炎症反应中发挥着作用。

Abstract:

Objective：To investigate expression of transcription factor activator protein-1（AP-1） in lumbar sympathetic ganglion neurons of patients with Buerger’s disease. Methods：Specimens of lumbar sympathetic ganglion of male patients with Buerger’s disease between January 2011 and March 2012 were collected and divided into two groups：Buerger’s disease group（n=23） and normal control group（n=12）. Immunohistochemistry，reverse transcription quantitative PCR and Western blot were used to detect and compare expression of AP-1 in lumbar sympathetic neurons of patients with and without Buerger’s disease. Results：AP-1 gene（P=0.045 9） and protein（P=0.028 3） expression in lumbar sympathetic ganglion neurons of patients with thromboangiitis obliterans were higher than those of normal people. Conclusions：AP-1 plays a role in the regulation of cell proliferation and vascular inflammation response in Buerger’s disease.