变应性鼻炎豚鼠模型中Th2/Th17相关细胞因子检测

Detection of concentrations of Th2/Th17 cytokines in guinea pig model of allergic rhinitis

摘要:

目的：分析变应性鼻炎（allergic rhinitis，AR）豚鼠模型鼻腔灌洗液白细胞介素-17（interleukin-17，IL-17）、白细胞介素-4（interleukin-4，IL-4）及鼻相关淋巴组织中白细胞介素-23（interleukin-23，IL-23）mRNA的表达水平，探讨该模型中是否存在Th2/Th17细胞因子失衡。方法：建立AR豚鼠模型，收集鼻腔灌洗液，采用ELISA法检测灌洗液中IL-17和IL-4，分离鼻相关淋巴组织，通过RT-PCR半定量检测IL-23 mRNA的表达水平，比较模型组和对照组间细胞因子水平差异。结果：AR模型组鼻腔灌洗液IL-17水平为[（4.98±1.54） pg/ml]，比对照组[（12.68±3.32） pg/ml]低，差异有统计学意义（t=10.35，P=0.006）。鼻相关淋巴组织中IL-23 mRNA的表达水平与IL-17的表达相似；而鼻腔灌洗液中IL-4的表达水平正好相反，与对照组 [（6.60±1.36） pg/ml]比较，模型组IL-4的表达水平[（31.20±4.25） pg/ml]高，差异有统计学意义（t=17.26，P=0.001）。结论：Th17相关的细胞因子IL-17、IL-23在AR动物模型中表达降低，而Th2细胞因子IL-4的表达水平明显升高，从而推测在AR中可能存在Th2/Th17细胞因子网络失衡，这种Th2/Th17细胞因子网络失衡可能对变应性炎症的发生起到关键的重要。

Abstract:

Objective：To investigate the changes of interleukin- 17（IL-17）and interleukin-4（IL-4） in nasal lavage fluid and inter－leukin-23（IL-23） mRNA in nasal associated lymphoid tissue of animal model of allergic rhinitis（AR）. Methods：Guinea pig model of AR was established. Nasal lavage fluid was collected，then IL-17 and IL-4 levels in nasal lavage fluid were measured by ELISA. Nasal associated lymphoid tissue was separated，then IL-23 mRNA expression level was detected by RT-PCR. Differences of cytokines level were compared between model group and control group. Results：Level of IL-17 in nasal cavity lavage fluid was （（4.98±1.54） pg/ml） in model group and （（12.68 ± 3.32） pg/ml） in the control group. Level of IL-17 decreased in model group than in control group with statistically significant differences（t=10.35，P=0.006）. mRNA level of IL-23 in nasal-associated lymphoid tissue was the same with IL-17. But the level of IL-4 in the nasal cavity lavage fluid（（31.20±4.25） pg/ml） was significantly higher in model group than in control group（（6.60±1.36） pg/ml），with statistically significant differences（t=17.26，P=0.001）. Conclusion：Levels of IL-17 and IL-23 decrease in AR model. But level and levels of IL-4 are significant higher in model group than in control group. Thereby we infer that there is the imbalance of Th2/Th17 cytokine network in AR，which may play an important role in allergic inflammation.