水黄花药材高效液相色谱指纹图谱及模式识别

High performance liquid chromatography fingerprint chromatogram and chemical pattern recognition method of Euphorbia chrysocoma Levl.etVant.

摘要:

目的：研究水黄花药材高效液相色谱（high performance liquid chromatography，HPLC）指纹图谱及模式识别方法。方法：采用HPLC，以贵州不同产地、不同采收期的水黄花药材为对象，对15批药材进行指纹图谱研究，运用聚类分析对指纹图谱进行模式识别研究。结果：15批样品中有13个共有峰，15批药材相似度较高，色谱峰强度不同是药材的主要差别。通过聚类分析将15批样品按采收期不同分为2类。结论：该方法能很好分离水黄花的各类成分，HPLC指纹图谱具有良好的稳定性、重复性，可用于水黄花药材的质量控制。

Abstract:

Objective：To study the high performance liquid chromatography（HPLC） fingerprint chromatogram and chemical pattern recognition method of Euphorbia chrysocoma Levl.etVant. Methods：Fifteen batches of Euphorbia chrysocoma Levl.etVant. samples were harvested at different time periods in different producing areas of Guizhou and were carried out to gain fingerprints by HPLC. Cluster analysis was conducted to do chemical pattern recognition of fingerprints of Euphorbia chrysocoma Levl.etVant.. Results：Fin－gerprints of 15 batches of samples demonstrated 13 characteristic peaks. Chromatographic profiles of 15 batches of samples were simi－lar；major differences resulted from intensity of chromatographic peaks. Samples were grouped into 2 types based on the harvest time. Conclusion：Components of Euphorbia chrysocoma Levl.etVant. can be well separated on HPLC chromatogram. HPLC fingerprint chromatogram has good repeatability and stability and can be used for quality control of Euphorbia chrysocoma Levl.etVant．