大鼠角膜缘新生血管微小RNA与血管内皮生长因子相关性分析

Correlation between microRNA expression profiles and VEGF of corneal neovascularization in rats

摘要:

目的：研究微小RNA（MicroRNA）在大鼠角膜缘新生血管中的表达，分析其与血管内皮生长因子（vascular endothelial growth factor，VEGF）血管生成信号通路间的相关性，探讨MicroRNA在角膜缘新生血管中的作用。方法：采用缝线法建立SD大鼠角膜缘新生血管模型，选择LAN MicroRNA芯片，采用酶标记方法标记样品，将样品与芯片探针杂交，对表达谱芯片结果进行分析。结果：明显差异表达MicroRNA为16个，其中上调10个，下调6个。明显上调的MicroRNA有：miR-142、miR-146、miR-21、miR-223、miR-214、miR-199、miR-483、miR-322、miR-126 miR-1224；明显下调的有miR-30e、miR-101、miR-184、miR-200c、miR-200b、miR-204。其中与VEGF信号通路密切相关的有：miR-142、miR-146、miR-21、miR-223、miR-199、miR-322、miR-200、miR-30e、miR-204、miR-1224。通过检索，miR-142 miR-200和miR-322与VEGF相关基因重合最多。结论：miR-142 miR-200和miR-322在大鼠角膜缘新生血管模型中差异表达显著且与VEGF信号通路密切相关。

Abstract:

Objective：To study differential microRNAs（miRNAs） expression profiles in rat corneal neovascularization（CNV） using mi－croarrays，to establish the potential link between microRNAs and vascular endothelial growth factor（VEGF） signaling pathways and to investigate the function of neovascularization in corneal limbus. Methods：Corneal neovascular model in rats was constructed by corneal stitch. The LAN microarrays were selected and samples were labeled and hybridized with microarray. The results of LAN microarrays were analyzed. Results：Sixteen miRNAs were predicted to target emcee of CNV including 10 miRNAs expressing as up-regulation and 6 miRNAs expressing as down-regulation. The miRNAs expressing as up-regulation included miR-142，miR-146，miR-21，miR-223，miR-214，miR-199，miR-483，miR-322，miR-126 and miR-1224. The miRNAs expressing as down-regulation included miR-30e，miR-101，miR-184，miR-200c，miR-200b and miR-204. The miRNAs correlated significantly with VEGF included miR-142，miR-146，miR-21，miR-223，miR-199，miR-322，miR-200，miR-30e，miR-204，miR-1224. miR-142 miR-200 and miR-322 correlated most with VEGF. Conclusion：Differential expression of miR-142 miR-200 and miR-322 in the rat model of corneal neo－vascularization is closely associated with VEGF signaling pathway.