电针对非酒精性脂肪肝大鼠脂代谢和炎症因子的干预效应

Interventional effect of electro-acupuncture on the lipid metabolism and inflammation factor of nonalcoholic fatty liver in rats

摘要:

目的：通过电针对非酒精性脂肪肝（nonalcoholic fatty liver disease，NAFLD）大鼠血脂、肝功、肝脏组织形态学、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白介素6（interleukin-6，IL-6）、白介素18（interleukin-18，IL-18）的炎症因子的影响，观察电针对NAFLD炎症的干预效应。方法：将48只雄性SD大鼠随机分成正常组、模型组、早期干预组、晚期干预组，每组各12只；模型组、早期干预组、晚期干预组予以高脂饲料喂养5周建立NAFLD模型，在5周末每组各处死１只老鼠，以验证造模成功。早期干预组在适应性喂养高脂饲料1周后给予电针干预，各干预组均电针足三里、丰隆、三阴交，每日1次，每次20 min，连续治疗21 d。观察大鼠血脂、肝功、肝脏组织形态学、炎症因子变化情况。结果：模型组谷草转氨酶、谷丙转氨酶、总胆固醇、甘油三酯、TNF-α、IL-6、IL-18水平明显高于其他各组，差异有统计学意义（F=103.235、148.117、7.496、6.343、11.402、16.498、11.525，P=0.000、0.000、0.000、0.001、0.000、0.000、0.000）；针刺后各干预组血脂、肝功、TNF-α、IL-6、IL-18的水平明显低于模型组，差异有统计学意义（P<0.05）。结论：电针可以通过改善NAFLD血脂和炎症反应达到治疗NAFLD的目的。

Abstract:

Objective：To observe the effect of electro-acupuncture（EA） on blood lipid，liver function，histology of liver tissue，tumor necrosis factor-α（TNF-α），interleukin-6（IL-6） and interleukin-18（IL-18） in rats with non-alcoholic fatty liver disease（NAFLD） so as to investigate effect of EA intervention on NAFLD. Methods：Totally 48 male Sprague Dawley rats were randomly divided into four groups：normal group，model group，early intervention group，late intervention group. Rats in normal group were fed with normal-fat diet. Rats in the other three groups were fed with high-fat diet for five weeks to form non-alcoholic fatty liver model. At the end of the fifth week，one rat in each group was killed to verify the forming of fatty liver model. Rats in early intervention group were treated with EA at one week after feeding with high fat diet .Rats in late intervention group and early intervention group were treated with EA at the acupoints of Zusanli，Fenglong and Sanyinjiao for 20 min once a day for a consecutive 21 d. In the wake of the 21st d，changes in glutamic-pyruvic transaminase（ALT），glutamic oxalacetic transaminase（AST），total cholesterol（TC），triglyceride（TG），TNF-α，IL-6，IL-18 of rats in each group were observed. Results：Expressions of ALT，AST，TC，TG，TNF-α，IL-6，IL-18 were all higher in model group than in the other three groups，with statistically significant differences（F=103.235，148.117，7.496，6.343，11.402，16.498，11.525；P=0.000，0.000，0.000，0.001，0.000，0.000，0.000）. Expressions of ALT，AST，TC，TG，TNF-α，IL-6，IL-18 were significantly reduced in two intervention groups than in model group，with statistical significances（P<0.05）. Conclusion：EA can treat NAFLD through alleviation of blood lipid and inflammation.