补中益气丸干预健康气虚质人群的血浆代谢组学研究

Plasma metabonomic analysis of healthy qi-deficiency constitution after the intervention by Buzhongyiqi pills

摘要:

目的：基于核磁共振（nuclear magnetic resonance，ＮＭＲ）的代谢组学方法，对气虚质健康人干预前后的血浆内源性代谢物的变化进行了比较研究，寻找气虚质的可能特征代谢物。方法：招募健康气虚质20人随机分为干预组和对照组，应用补中益气丸进行1个月的干预，并对他们的血浆样品进行代谢组学检测，采用多元统计分析方法寻找补中益气丸干预气虚质血浆内源性差异代谢产物。结果：对补中益气丸干预前后血浆样本应用Simca-P软件分析提取变量重要性预测值（variable importance predictive，ＶＩＰ），筛选出VIP>1，P<0.05的有差异的化学位移值45个，通过代谢产物数据库发掘对分类贡献较大的特异性代谢物30个。结论：这些差异代谢产物主要与三羧酸循环相关，说明补中益气丸调节了气虚质的能量代谢异常。而补中益气丸干预后疲劳与记忆力下降等症状的改善则可能与其能调节伽马氨基丁酸、磷脂酰胆碱、血氨的浓度有关。

Abstract:

Objective：To compare the endogenous metabolites in the plasma of people with qi-deficiency constitution after the inter－vention by Buzhongyiqi pills using nuclear magnetic resonance（NMR）-based metabonomics and to find the possible specific metabo－lites. Methods：Twenty healthy people with qi-deficiency constitution were recruited and divided into control group and intervention group. Buzhongyiqi pills were used to do the intervention and the plasma was tested by NMR-based metabonomics before and after the intervention. Multivariate statistical analysis was done to find the serum-derived metabolic differences after the intervention by Buzhongyiqi pills. Results：The plasma before and after the intervention was analyzed by Simca-P and 45 changed chemical shift val－ues（VIP>1，P<0.05） were selected. Thirty specific metabolites were found through human metabolite database. Conclusion：These differences in metabolites are related to the tricarboxylic acid cycle. Buzhongyiqi pills can improve the disorder of energy metabolism，memory deterioration and fatigue of qi-deficiency population，which may associated with its regulation of GABA，phosphatidyl－choline，blood ammonia concentration.