分枝杆菌噬菌体Chy3对人气道上皮细胞16HBE生长及功能的影响

Effects of mycobacteriophage Chy3 on growth and function of human airway epithelial cell 16HBE

摘要:

目的：探讨不同滴度的分枝杆菌噬菌体 Chy3对人气道上皮细胞16HBE生长及功能的影响。方法：不同滴度噬菌体 Chy3作用于人气道上皮细胞后，采用倒置显微镜观察细胞生长形态变化，CCK-8法检测细胞存活率，流式细胞仪检测细胞凋亡率，ELISA法检测细胞上清液中细胞因子分泌水平，RT-PCR和 Western blot分别检测上皮细胞黏蛋白 MUC5AC mRNA和蛋白表达水平。结果：不同滴度噬菌体 Chy3作用于人气道上皮细胞一段时候后，细胞的生长形态、存活率、凋亡率、细胞因子分泌水平以及黏蛋白 MUC5AC表达与正常组比较差异均无统计学意义（P ＞0.05）。结论：分枝杆菌噬菌体 Chy3无论高低滴度对体外培养人气道上皮细胞生长和功能均无明显影响，有较好的安全性。

Abstract:

Objective：To explore the effects of different titers of mycobacteriophage Chy3 on growth and function of human airway ep－ithelial cell 16HBE. Methods：Inverted microscope was used to observe cellular morphology after applying different titers of mycobac－teriophage Chy3. Cell viability and apoptosis rates were analyzed by CCK-8 assay and flow cytometry. Additionally，the secretion lev－els of IL-6/IL-8 in cell culture supernatant were detected by ELISA. RT-PCR and Western blot were performed to detect MUC5AC mRNA and protein expression. Results：There was no difference in cellular morphology，cell viability，apoptosis rate，secretion levels of IL-6/IL-8，MUC5AC mRNA and protein expression between cells treated with different titers of Chy3 and normal controls（P ＞0.05）. Conclusion：Neither high nor low titer of mycobacteriophage Chy3 exerts effects on growth and function of human airway epithelial cell 16HBE in vitro，therefore mycobacteriophage has better security.