反相高效液相色谱法测定柚皮苷纳米纤维膜中柚皮苷含量

Determination of naringin in nanofiber membranes by reversed-phase high-performance liquid chromatographic

摘要:

目的：建立能有效测定柚皮苷纳米纤维膜中柚皮苷含量的反相高效液相色谱法。方法：采用静电纺丝技术制备含有柚皮苷的纳米纤维膜样品，采用安捷伦C18色谱柱（4.6 mm×150 mm，5 μm），流动相为乙腈-磷酸水（pH 2.8）（23∶77），检测波长为283 nm，流速为1.0 ml/min，柱温为30 ℃，进样体积为20 μl。结果：空白纳米纤维膜对柚皮苷的检测无干扰，专属性良好。柚皮苷最低检测限S/N=3为3.4 ng/ml；定量限 S/N=10为10.2 ng/ml；在51 ng/ml～1 836 μg/ml范围内呈良好的线性关系（相关系数r=0.999 99）；日内和日间精密度以及重现性均良好；平均回收率为99.51％，相对标准偏差=0.66％；检测溶液在24 h内稳定。结论：柚皮苷纳米纤维膜是采用静电纺丝技术制备的含有柚皮苷的纳米级纤维膜，是一种新型的载药引导性骨再生膜。本法简便、准确、快速，可用于柚皮苷纳米纤维膜中柚皮苷的含量测定，为研究其最佳载药量打下基础。

Abstract:

Objective：To establish a reversed-phase high-performance liquid chromatographic method to determine the content of naringin in nanofibers. Methods：The test film，nanofiber film containing naringin，was prepared using electrospinning equipment. The determination was performed on Agilent C18 chromatographic column（4.6 mm×150 mm，5 μm）. The acetonitrile-phosphoric water （pH 2.8） （23∶77） was used and the detecting wavelength was 282 nm. The column temperature was 30 ℃. The sampling inlet vol－ume was 20 μl. Results：Pure PCL nanofiber，having good specificity exerted no interfering effect on naringin determination. The low－est detection limit of naringin S/N=3 was 3.4 ng/ml. The limit of quantitation S/N=10 was 10.2 ng/ml. Good linear dependence（corre－lation coefficient r=0.999 99） was detected when the concentration of naringin was within the range of 51-1 836 μg/ml. This method had good precision and repeatability both within and between days. The average recovery of naringin was 99.51% and the relative standard deviation was 0.66%. The test solution was stable within 24 h. Conclusion：Naringin nanofiber film can be produced using electrospinning technique which is a nano film with medicine loaded for bone regenerative. This study provides an easy，accuracy，rapid way for the quantitative determination of naringin in nanofibers and solid basis for the evaluation of the optimum content of naringin in nanofibers.