家兔腹壁疝模型建立及其判定标准的探索研究

Construction of rabbit abdominal hernia model and its criteria

摘要:

目的：通过动物实验研究探索出1种能够建立稳定动物腹壁疝模型方法的可行性以及判定其成功的标准。方法：将40只雄性家兔完全随机分为A、B、C、D 4组，每组10只，A组为空白对照组，其余3组为实验组，分别切除2 cm×2 cm、3 cm×3 cm、4 cm×4 cm全层腹壁组织结构，1个月后观察各组动物腹壁突出情况、动物体质量变化及死亡情况。结果：1个月后各组动物体重A、B、C 3组较术前无差异（P值分别为0.504，0.465，0.090），D组动物体质量较术前减轻（P=0.000），且较其他组体质量变化量大（P=0.000）；A组动物无腹壁包块突出，无死亡例数，B组2例，1例死亡，C组6例，1例死亡，D组6例，3例死亡。结论：切除腹壁3 cm×3 cm全层腹壁组织结构（肌肉和腹膜）可以得到稳定的动物疝模型，并提出１种成功建立疝模型的方法及判定标准，为未来疝相关研究提供了稳定的动物模型。

Abstract:

Objective：To explore the feasibility of establishing a stable animal model of abdominal hernia and the criteria to determine its success through animal studies. Methods：The 40 male rabbits were randomly divided into A，B，C and D groups with 10 rabbits in each group. A group was taken as control group and the remaining three groups were taken as experimental groups. Full structure of abdominal wall（muscle and peritoneum）of rabbits in experimental group was cut by sizes of 2 cm×2 cm，3 cm×3 cm，4 cm×4 cm，respectively. Protruding abdominal mass，body weight and mortality of rabbits were observed one month later. Results：There was no significant difference in body weight in groups A，B，C before and after the surgery（P=0.504，0.465，0.090）. Body weight was decreased in group D after the surgery compared with that before the surgery（P=0.000）. No case of abdominal mass and no death case in group A，two cases of abdominal mass and one death case in group B，six cases of abdominal mass and one death case in group C，six cases of abdominal mass and three death cases in group D were observed. Conclusion：Removal of abdominal tissues by size of 3 cm×3 cm can build a stable animal model of abdominal hernia and propose a method to build hernia model and criteria，paving the way for the research of the hernia in the future.