脂质纳米粒提高尿酸氧化酶体外稳定性的初步研究

Preliminary study on the stability of urate oxidase loaded in lipid nanoparticles

摘要:

目的：使用Tricine-NaOH缓冲液制备含UOX的脂质纳米粒（urate oxidase lipid nanoparticles prepared by Tricine-NaOH buffer，UTLNs），并对游离尿酸氧化酶（urate oxidase，UDX）在体外的活性和稳定性进行初步研究。方法：采用逆向蒸发法制备UTLNs。分别从最适温度、最适pH、热稳定性、贮存稳定性、酸碱稳定性和抗胰蛋白酶水解能力初步考察游离UOX和UTLNs的差异。结果：游离UOX和UTLNs最适温度均为40 ℃；游离UOX最适pH为8.5，UTLNs最适pH为8.0；稳定性结果显示，UTLNs的体外稳定性明显优于游离UOX。结论：UTLNs不仅提高了UOX在体外的活性，并且明显地增强了UOX在体外的稳定性。

Abstract:

Objective：To prepare urate oxidase lipid nanoparticles by Tricine-NaOH buffer（UTLNs） and then to study the in vitro ac－tivity and stability of free UOX and UTLNs. Methods：UTLNs was prepared by reverse-phase evaporation method. The optimum tem－perature，optimum pH，thermal stability，storage stability，pH stabilities and proteolytic stabilities of free UOX and UTLNs were mea－sured. Results：The optimum temperatures for free UOX and UTLNs were both 40 ℃. The optimum pH for free UOX and UTLNs were 8.5 and 8.0，respectively. UTLNs had significantly better stability than free UOX in vitro. Conclusion：UTLNs not only improved the activity but also significantly enhanced the stability of free UOX in vitro.