大鼠同种异体肾移植急性排斥反应蛋白质组学研究

Proteome analysis of renal allograft acute rejection in rats

摘要:

目的：探讨大鼠肾移植术后移植肾急性排斥反应蛋白质组学变化，以期寻找新的生物学标志物。方法：实验组以近交系雄性F344和Lewis大鼠分别作为供体和受体，共8对。对照组以近交系雄性F344大鼠作为供体和受体，共8对。分别建立大鼠肾移植模型，术后7 d获取移植肾组织，行双向凝胶电泳（two-dimensional electrophoresis，2-DE）和基质辅助激光解析电离飞行时间质谱（matrix-assisted laser desorption ionization-time of flight mass spectrometry，MALDI-TOF-MS）分析，筛选并鉴定差异表达蛋白。结果：实验组和对照组蛋白斑点数量分别为（554.0±34.0）和（542.0±21.0）个。实验组与对照组比较，37个蛋白点的表达水平发生显著变化，其中29个点得到较满意的肽质量指纹图，在SwissProt数据库中进行搜索、鉴定出6个差异的蛋白。结论：本实验通过比较蛋白质组学方法成功筛选并鉴定出6个可能与大鼠移植肾急性排斥反应有关的差异表达蛋白，并可能成为移植肾急排新的生物学标志物。

Abstract:

Objective：To analyze changes of protein expression in rat transplant kidney by proteomics technique and to search and identify differential expression proteins which are related with acute rejection. Methods：All transplant rats were divided into two groups： experimental group（F344→Lewis） and control group（F344→F344）. Rat models of allogene renal transplantation were estab－lished respectively and allograft specimens were obtained on 7 d post the transplantation. Total proteins of rat transplant renal tissues were separated by two-dimensional gel electrophoresis（2-DE）. Differential expression proteins were analyzed and then identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry（MALDI-TOF-MS）. Results：Averages protein spots in ex－perimental group and control group were 554.0±34.0 and 542.0±21.0 respectively. Compared with those in control group，37 dif－ferentially expressed proteins were screened in experimental group，of which 29 were identified by peptide mass fingerprint and 6 were founded by bioinformatics in SwissProt data base. Conclusion：Six differential expression proteins which may correlate with acute rejection of renal allograft are identified by comparative proteomics and might become possible biological marker of acute rejection of renal allograft.