不同灸法对部分雄激素缺乏综合征大鼠性激素/促性激素分泌调节及SOD、MDA影响的研究

Effects of different moxibustion therapies on contents of SOD，MDA and reproductive endocrines in male rats with partial androgen deficiency

摘要:

目的：观察不同灸法对部分雄激素缺乏综合征大鼠性激素/促性激素分泌调节以及超氧化物歧化酶（superoxide dismu－tase，SOD）、丙二醛（malonic dialdehyde，MDA）的影响。方法：将50只雄性SD大鼠随机分成2组：正常组（n=10）和模型组（n=40），模型组腹腔注射环磷酰胺中老年男性部分雄激素缺乏综合征（partial androgen deficiency of aging male，PADAM）大鼠模型。从造模成功的大鼠中随机选取32只平均分为4组：温和灸组、瘢痕灸组、药物组和对照组。于造模前、造模后和治疗后测定大鼠血清总睾酮（total testosterone，TT）、游离睾酮（free testosterone，FT）值及悬尾和强迫游泳时间。于治疗后测定血清黄体生成素（luteinizing hormone，LH）、卵泡刺激素（follicle-stimulating hormone，FSH）水平及肾脏、睾丸匀浆SOD、MDA含量。结果：（1）运用环磷酰胺复制PADAM大鼠模型成功。（2）治疗结束后，温和灸组、瘢痕灸组、药物组血清TT、FT含量较治疗前及对照组明显提高（P值均为0.000）；血清LH、FSH含量较对照组明显下降（P值均为0.000）；悬尾和强迫游泳时间明显改善（P值均为0.000）；肾脏、睾丸SOD含量明显高于对照组（P值均为0.000）而MDA含量明显低于对照组（P值均为0.000）；温和灸组与瘢痕灸组、药物组相比较疗效更明显（P=0.004，P=0.009；P=0.000，P=0.000；P=0.000，P=0.003），瘢痕灸组、药物组比较无统计学差异（P=0.762，P=0.282，P=0.247）。结论：（1）2种灸法均可提高PADAM大鼠血清TT、FT水平及肌张力和抗疲劳能力，其作用与丙酸睾丸素相当。（2）2种灸法均可提高PADAM大鼠体内SOD而降低MDA，提示灸法治疗PADAM可能与其改善体内自由基系统有关联。（3）温和灸疗效更佳，为临床灸法选择提供实验依据。

Abstract:

Objective：To observe the effects of different moxibustion therapies on contents of superoxide dismutase（SOD），malonic dialdehyde（MDA） and reproductive endocrine in male rats with partial androgen deficiency of aging male（PADAM）. Methods：Totally 50 male rats were randomly divided into normal control group（n=10） and model group（n=40）. The rats in model group were injected with cyclophosphamide in abdominal cavities to make PADAM model. Thirty-two rats were randomly selected from model group and were randomized into mild-moxibustion group，scarring-moxibustion group，rolin group and control group. Before modeling，after modeling and at the end of experiments，all rats' serum total testosterone（TT） and free testosterone（FT） levels were detected and exhaustion swimming tests and tail suspension experiments were observed. The SOD and MDA levels in kidney and testis tissues as well as the serum luteinizing hormone（LH） and follicle-stimulating hormone（FSH） levels were determined at the end of the experiments. Results：（1）A successful modeling of PADAM was made by using cyclophosphamide. （2）After the treatments，the serum TT and FT levels in three treatment groups increased signifi－cantly compared with those pre-therapy and control group（P=0.000）. The serum LH and FSH levels decreased significantly（P=0.000）. The duration of immobility and the duration of ex－haustion swimming also improved significantly（P=0.000）. SOD activities in the kidney and testis increased obviously（P=0.000） and MDA contents decreased significantly（P=0.000）. The curative effect of mild-moxibustion group was better compared with that of the other two groups（P=0.004，P=0.009；P=0.000，P=0.000；P=0.000，P=0.003） and no significant difference was found between scarring-moxibustion group and androlin group（P=0.762，P=0.282，P=0.247）. Conclusion：（1）Two kinds of moxibustion therapies could improve the depression state，myodynamia and energy in the aging rats as well as increase TT and FT levels，with the similar effects of testosterone propionate. （2）Two kinds of moxibustion therapies could increase SOD activities and decrease MDA contents in the kidney，indicating a correlation between electro-acupuncture cured PADAM and improved free radical related systems in the body. （3）Mild-moxibustion had a better curative effect，providing experi－mental data and proof for clinical treatment.