生精相关基因Znf230在小鼠睾丸及精子中的表达和定位研究

Expression and localization of the spermatogenesis-related gene Znf230 in mouse testis and spermatozoa

摘要:

目的：应用小鼠Znf230多克隆抗血清，对Znf230在小鼠睾丸及精子中的表达及定位进行研究。方法：用RT-PCR分析、Western blot检测、免疫组织化学和免疫荧光染色方法研究Znf230在小鼠不同发育阶段睾丸组织及精子中的表达及亚细胞定位情况。结果：小鼠从出生到成年的发育过程中，Znf230的表达水平基本上是稳定的。小鼠出生6 d和12 d时，该蛋白在精原细胞核中表达，从出生18 d到精子发育成熟这个阶段，该蛋白转移到圆形精子细胞、延长精子细胞和精子中表达，具体定位于顶体区域和精子的尾部。结论：Znf230可能在小鼠生精过程中起着重要的作用。

Abstract:

Objective：To detect the expression and location of Znf230 in mouse testis and spermatozoa by Znf230 polyclonal anti－serum. Methods：Expression and localization of Znf230 in mouse testis at different developmental stages and epididymal spermato－zoa were determined by RT-PCR，immunoblotting，immunohistochemistry and immunoluorescence. Results：Znf230 remained relatively stable and was expressed throughout postnatal development of the mouse testis. It was primarily expressed in the nuclei of spermato－gonia（from postnatal d 6 to postnatal d 12） and subsequently in the acrosome system and the entire tail of round spermatids，elongated spermatids and spermatozoa（from postnatal d 18 to mature sperm）. Conclusion：Znf230 may play an important role in mouse spermatogenesis.