转化生长因子β1在环磷酰胺导致大鼠生精细胞凋亡中的表达及意义

Expression and significance of transforming growth factor-β1 in the apoptosis of rat spermatogenic cells induced by cytoxan

摘要:

目的：初步探讨环磷酰胺（cytoxan，CTX）导致生精细胞凋亡中睾丸组织内转化生长因子β1（transforming growth factors β1，TGF-β1）的表达及意义。方法：将CTX作用于9 d龄雄性Wistar大鼠，用药后24 h、4周、8周实验组和对照组分别取材10只，采用TUNEL法检测生精细胞凋亡，采用RT-PCR技术检测睾丸组织中TGF-β1表达。结果：（1）用药后24 h实验组较对照组生精细胞凋亡无明显差异（F组间=1.25，P=0.924），TGF-β1-mRNA表达无明显差异（F组间=1.59，P=0.339）。（2）用药后4周、8周实验组生精细胞凋亡较对照组明显增多（F组间=126.73，P=0.005），实验组TGF-β1-mRNA表达明显大于对照组（F组间=32.86，P=0.007）。结论：CTX可增加生精细胞凋亡，且伴有TGF-β1表达明显增加，提示CTX引起TGF-β1表达增加可能导致生精细胞凋亡增多。

Abstract:

Objective：To preliminarily assess the expression and significance of transforming growth factor-β1（TGF-β1） in the apop－tosis of rat spermatogenic cells induced by cytoxan. Methods：Cytoxan was applied for male Wistar rats aged 9 d. After 24 h，4 weeks and 8 weeks，apoptosis of spermatogonia was counted severally by TUNEL and TGF-β1 was detected severally by RT-PCR in testes of 10 rats in experimental group and control group. Results：（1）There was no difference in the apoptosis of spermatogenic cell and TGF-β1 expression between control group and experimental group at 24 h after the treatment at 24 h after the treatment. （Fbetween groups=1.25，P=0.924）（Fbetween groups=1.59，P=0.339）（2）Apoptosis of spermatogenic cell and TGF-β1 expression were significantly increased in experimental groups than in control group（Fbetween groups=126.73，P=0.005）（Fbetween groups=32.86，P=0.007）. Conclusion：Cytoxan not only increases apoptosis of spermatogenic cell in testes，but also results in elevated expression of TGF-β1 of testes in rat，indicating that increased expression of TGF-β1 in testes will lead to increased apoptosis of spermatogenic cell in testes.