促肝细胞再生磷酸酶1基因对舌癌细胞侵袭的影响

Effects of phosphatase of regenerating liver cell-1 on invasion of human tongue cancer cells

摘要:

目的：探讨促肝细胞再生磷酸酶1（phosphatase of regenerating liver cell-1，PRL-1）对舌癌细胞侵袭能力的影响及其可能的机制。方法：应用 siRNA-PRL-1慢病毒载体转染处理舌癌 TCA8113细胞株后，分别采用荧光定量PCR和蛋白质印迹检测PRL-1基因和基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）、基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9） mRNA和蛋白水平；采用 Transwell小室模型实验检测癌细胞的侵袭能力。结果：siRNA-PRL-1慢病毒转染组癌细胞PRL-1在RNA（0.425 0±0.010 0）和蛋白（2.720 0±0.110 0）水平明显下调（P=0.000，P=0.000），同时MMP-2及MMP-9在RNA（0.562 2±0.180 0，0.617 1±0.100 0）及蛋白（0.592 4±0.010 0，0.476 2±0.020 0）表达水平降低（P=0.024，P=0.010，P=0.000，P=0.000）；Transwell实验siRNA-PRL-1慢病毒转染组细胞通过数（64.33±4.04）明显减少（P=0.000）。结论：siRNA-PRL-1转染可抑制舌癌细胞侵袭，其机制可能与PRL-1基因下调MMP-2及MMP-9的表达有关。

Abstract:

Objective：To study the effects of phosphatase of regenerating liver cell-1（PRL-1） small interfering RNA（siRNA）on inva－sion of tongue cancer cells（TCA8113） and the mechanism. Methods：After tongue squamous cancer cell line TCA8113 being trans－fected by PRL-1 siRNA，the mRNA and protein of PRL-1 and matrix metalloproteinase-2（MMP-2） and matrix metalloproteinase-9 （MMP-9） were determined by real-time PCR and Western blot assay，respectively. TCA8113 cell invasiveness was detected through Transwell chamber using model test. Results：After transfection by PRL-1 siRNA，the level of mRNA（0.425 0±0.010 0） and pro－tein（2.720 0±0.110 0） of PRL-1 was down-regulated（P=0.000，P=0.000）. The mRNA level of MMP-2 and MMP-9（0.562 2±0.180 0，0.617 1±0.100 0）as well as the protein level of MMP-2 and MMP-9（0.592 4±0.010 0，0.476 2±0.020 0） were decreased obviously（P=0.024，P=0.010，P=0.000，P=0.000）. The invasion ability of human tongue squamous cancer cell was reduced（64.33±4.04）（P=0.000）. Conclusion：PRL-1 gene might play an important role in development of human oral squamous cell carcinoma and down-regulation of PRL-1 could inhibit invasion of human tongue squamous cancer cell. This may be related to the lower expression of MMP-2 and MMP-9.