目的：研究大鼠实验性牙周炎在发生发展过程中，糖尿病对其的发挥的作用及影响。方法：选取48只雄性SD大鼠采用在右上颌第一磨牙牙周拴丝的方法构建大鼠牙周炎模型。随机均分为２组，每组24只，糖尿病+牙周炎组（G1组），牙周炎组（G2组）。G1组大鼠通过在腹腔内注射链脲佐菌素（streptozotocin，STZ）建立糖尿病模型。分别在建模后第1、2、3、4周每组（G1组，G2组）随机处死6只大鼠，测量龈沟出血指数（sulcular bleeding index，SBI），牙周探诊深度（probing depth，PD），牙槽骨丧失高度（alveolar bone loss，ABL），进行牙周形态学的观察和免疫组化检测牙周组织中白介素1β（interleukin-1β，IL-1β）、肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）的表达。结果：（1）在同一时间，SBI、PD和ABL G1组>G2组，组间比较具有统计学差异（P<0.05）；（2）G1组牙周组织形态炎症表现较G2组严重；（3）在同一时间点，牙周组织中IL-1β、TNF-α的表达，G1组>G2组，组间比较有统计学意义（P<0.05）。结论：本实验条件下，糖尿病可以使大鼠牙周炎病变进一步恶化。
Abstract:
Objective：To research the effects of diabetes on periodontitis model in rats. Methods：The animal model of periodontitis was established by ligating the right maxillary first molar of 48 male SD rats（6 weeks，160-180 g） through stainless steel wire. All rats were divided into two groups randomly：periodontitis combined with diabetes（G1）group and periodontitis（G2） group. Diabete’s models were established by injecting streptozocin intraperitoneally. Six rats were killed in each group after 1，2，3，4 weeks. Sulcus bleeding index（SBI），probing depth（PD）and alveolar bone loss（ABL） were measured. Periodontal histology was observed by micro－scope. Interleukin-1β（IL-1β） and tumor nectosis factor-α（TNF-α） levels were analyzed by immunohistochemistry. Results：（1）At the same time，SBI，PD，ABL were higher in G1 group than in G2 group with statistical significances（P<0.05）；（2）The inflammations of periodontal histology were more serious in G1 group than in G2 group；（3）At the same time，IL-1β and TNF-α levels were higher in G1 group than in G2 group（P<0.05）. Conclusion：Under the experiment condition，diabetes can aggravate the rat periodontitis.
