高尔基体基质蛋白130、14-3-3ζ、整合素α3在中、低分化胃癌组织的表达及意义

Expression and significance of GM130，14-3-3ζ and integrin α3 in moderately differentiated and poorly differentiated gastric cancer tissues

摘要:

目的：检测高尔基体基质蛋白130（Golgi matrix protein 130，GM130）、14-3-3 zeta（14-3-3ζ）、整合素α3（integrin al－pha3，Integrin α3）在正常胃组织和中、低分化胃癌组织中的表达，并由此探讨其与胃癌的关系。方法：运用免疫组织化学检测（strept avidin-biotin complex，SABC）法分别检测临床收集的84例胃癌患者的胃癌组织和正常胃组织（距癌肿边缘5 cm以上的胃组织）GM130、14-3-3ζ、Integrin α3的表达情况，并结合临床病理资料进行分析；用RT-PCR和Western blot分别检测42例胃癌（低分化胃癌24例，中分化胃癌18例）和42例正常胃组织GM130、14-3-3ζ、Integrin α3的mRNA和蛋白表达情况。结果：（1）ＧＭ1301、14-3-3ζ2、Integrin α33在胃癌组织中的表达阳性率分别为88.1%、90.5%、95.2%，明显高于上述各指标在正常胃组织上的阳性表达率（52.4%、27.4%、42.9%）；低分化胃癌组上述３个指标的表达较中分化胃癌组高，且２组间差异有统计学意义（P1=0.000、P2=0.007、P3=0.000）。Spearman等级相关性分析显示，胃癌中ＧＭ130、14-3-3ζ、Integrin α3之间表达呈两两正相关（P＜0.05）。（2）ＧＭ130、14-3-3ζ、Integrin α3的表达均与年龄、性别、肿瘤部位、肿瘤直径、肿瘤浸润深度、有无淋巴结转移无关（P >0.05）；而与肿瘤病理组织学分级及临床分期有关（P<0.05）。（3）RT-PCR及Western blot结果显示ＧＭ130、14-3-3ζ、Integrin α3 mRNA和蛋白质的表达在胃癌组较正常胃组织组高，低分化胃癌组较中和分化胃癌组高，差异有统计学意义（P<0.05）。结论：GM130的异常表达可能在胃癌的发生发展中发挥重要作用。

Abstract:

Objective：To detect the different expressions of GM130，14-3-3ζ and integrin α3 in normal gastric tissues，moderately differentiated gastric cancer tissues and poorly differentiated gastric cancer tissues thus to explore its relationship with gastric cancer. Methods：The expression of ＧＭ130，14-3-3ζ and integrin α3 in gastric carcinoma tissues of 84 patients with gastric cancer and the 84 samples of normal gastric tissues（from 5 cm above the edge of the gastric tumor tissues） were detected by SABC immunohisto-chemistry. The expression of proteins and the clinical pathological data were analyzed. The expression of mRNA and protein of ＧＭ130，14-3-3ζ and integrin α3 were detected by RT-PCR and Western blot. Results：The positive expression rates of ＧＭ1301，14-3-3ζ2 and integrin α33（88.1%，90.5%，95.2%） were significantly higher in gastric cancer tissues than those in normal gastric tissues（52.4%，27.4%，42.9%）；the expression of the above three indicators in poorly differentiated gastric cancer group were higher than those in moderately differentiated gastric cancer group，with statistically significant differences（P1=0.000，P2=0.007，P3=0.000）. Spearman correlation analysis showed that positive correlation were observed in ＧＭ130，14-3-3ζ and integrin α3 in gastric cancer（P＜0.05）. The expression of ＧＭ130，14-3-3ζ and integrin α3 in gastric cancer was obviously related with pathological differentiation and clinical stage（P<0.05），but its correlation with age，gender，tumor location，tumor size infiltration depth or whether the lymph node metastasis was not found（P ＞0.05）. The levels of mRNA and proteins were higher in gastric cancer group than in normal gastric tissue group，higher in poorly differentiated gastric cancer than in moderately differentiated gastric cancer，with statistically significant differences between the two groups（P＜0.05）. Conclusion：The abnormal expression of GM130 may play an important role in the occurrence and development of gastric cancer.