载多西紫杉醇脂质微泡联合超声靶向微泡破裂对人肝癌细胞增殖和凋亡影响的初步研究

Influence of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on proliferation and apoptosis of hepatocellular carcinoma cell

摘要:

目的：初步研究载多西紫杉醇脂质微泡（docetaxel-loaded lipid microbubbles，LDLM）联合超声靶向微泡破裂（ultrasound targeted microbubbles destruction，UTMD）对人SMMC-7721肝癌细胞增殖和凋亡的影响。方法：体外培养人SMMC-7721细胞，确定适宜的多西紫杉醇浓度，细胞分为6组，比较各组的细胞增殖抑制率、超微结构改变、凋亡率和细胞周期分布。结果：载药微泡+超声组的细胞增殖抑制率明显高于其他组，与其他各组相比差异有统计学意义（P=0.000）；电镜下观察载药微泡+超声组的凋亡细胞最多；流式细胞仪检测结果显示载药微泡+超声组的细胞凋亡率最高（P=0.000），LDLM+US组G0/G1期细胞比例明显减少，为（0.79±0.27）%（P=0.000），G2/M期细胞比例升高最明显，为（90.54±0.48）%（P=0.000）。结论：LDLM联合UTMD可抑制人SMMC-7721细胞的增殖、促进其凋亡，为后续进一步从蛋白、基因层面探索其作用的机制奠定了重要基础。

Abstract:

Objective：To study the influence of docetaxel-loaded lipid microbubbles（LDLM） combined with ultrasound targeted mi－crobubbles destruction（UTMD） on proliferation and apoptosis of SMMC-7721 cells. Methods：Suitable concentration of docetaxel was chosen. SMMC-7721 cells were cultured in vitro and divided into 6 groups. Proliferating inhibition rate，ultrastructural change，apop－totic rate and cell cycle distribution were compared among all groups. Results：The proliferating inhibition rate and apoptotic rate of LDLM+US group were the highest compared with that of other groups（P=0.000，P=0.000）. Great amount of apoptotic cells were observed in LDLM+US group by electron microscope and flow cytometry. Proportion of cells at G0/G1 phase was decreased obviously（0.79±0.27）%（P=0.000） while that at G2/M phase was obviously increased（90.54±0.48）%（P=0.000）. Conclusion：LDLM combined with UTMD could inhibit the proliferation and promote the apoptosis of SMMC-7721 cells，which provide evidence for the further study of its mechanisms in protein and gene level.