miRNA205启动子甲基化与Barrett食管癌变关系的体外研究

In vitro study of relationship between miRNA205 promoter methylation and Barrett’s esophagus cancerous

摘要:

目的：探讨microRNA205（miR205）启动子甲基化与Barrett食管（Barrett’s esophagus，BE）癌变的关系。方法：运用RT-PCR检测经去甲基化处理前后BE、食管癌和正常食管粘膜细胞中miR205表达水平，采用甲基化特异性聚合酶链式反应（methylation specific polymerase chain reaction，MSP）检测样本中miR205启动子甲基化水平。结果：BE和食管癌细胞miR205表达量较正常食管明显减低（P=0.002）；去甲基化处理后，样本miR205表达量水平明显升高（P=0.000）。MSP结果显示BE和食管癌细胞miR205启动子经去甲基化处理后呈低甲基化或非甲基化。结论：miR205在BE和食管癌细胞中表达降低与其启动子甲基化有关，miR205启动子甲基化贯穿了BE的癌变过程，有可能成为BE癌变过程中的关键分子生物学标志，并可能成为预防和治疗BE癌变的靶点。

Abstract:

Objective：To explor the relationship between MiR205 promoter methylation and Barrett’s esophagus cancerous. Methods：The expression of miRNA205 in Barrett’s esophagus，esophageal cancer and normal esophageal mucosa cells before and after demethylation processing was detected by RT-PCR. MiR205 promoter methylation levels were measured by methylation specific polymerase chain reaction（MSP）. Results：miR205 expression was reduced obviously in Barrett’s esophagus and esophageal cancer cells than in normal esophageal，with statistically significant differences（P=0.002）. After demethylation，miR205 expression was sig－nificantly increased in Barrett’s esophagus（BT，B-T10） and esophageal cancer（EC109，TE-10，SEG-1） cells，with statistically sig－nificant differences（P=0.000）. MSP showed miR205 promoter changes to be low-methylation or non-methylation. Conclusion：Re－duced expression of miR205 in Barrett’s esophagus and esophageal cancer cells is related to its promoter methylation. Throughout the carcinogenesis of Barrett’s esophagus，miR205 promoter methylation may become a key molecular biomarker in process of Barrett’s esophagus canceration and may become the prevention and treatment targets of Barrett’s esophagus canceration.