过氧化氧化还原蛋白2在结直肠癌中的表达及其作用机制的研究

Expression of PRDX2 in colorectal neoplasms and its mechanism

摘要:

目的：探讨过氧化氧化还原蛋白2 （peroxiredoxins 2，PRDX2）在结直肠癌及正常结直肠组织中的表达情况及其可能的作用机制。方法：收集32例组织标本，采用免疫组织化学及Western blot的方法对PRDX2在结直肠癌组织及正常结直肠组织中的表达情况进行检测；免疫共沉淀及HPLC-CHIP-MS/MS方法检测结肠癌细胞SW480中与PRDX2相互作用的蛋白质。结果：免疫组织化学显示：PRDX2蛋白主要表达于细胞浆，在结直肠正常组织（21.88%，7/32）与结直肠癌组织中（71.88%，23/32）PRDX2蛋白的表达差异有统计学意义（P=0.001）。随机抽取8例标本进行Western blot的检测，PRDX2蛋白在7例癌组织中表达高于正常组织，差异有统计学意义（P<0.01）。在结肠癌细胞SW480中鉴定出17种与PRDX2相互作用的蛋白质，其中有9种蛋白受c-Myc基因的调控。结论：PRDX2在结直肠癌的发展、转移中发挥了一定的作用；PRDX2可能与c-Myc基因调控通路间存在密切的联系。

Abstract:

Objective：To investigate the expression of PRDX2 in histological samples of colorectal neoplasm and the normal tissues and to analyze its mechanism. Methods：The expression of PRDX2 was detected by immunohistochemistry and Western blot. In colon cancer cell SW480，interacting proteins with PRDX2 was detected by immunoprecipitation and HPLC-CHIP-MS/MS. Results：Im－munohistochemistry indicated that PRDX2 protein was expressed mainly in the cytoplasm. The expression of PRDX2 was weaker in normal colorectal tissues（21.88%，7/32） than in carcinomas tissue（71.88%，23/32）（P=0.001）. Western blot analysis revealed that the expression of PRDX2 protein was significantly higher in carcinomas than in control colorectal tissues（P<0.01）. In SW480 cells，17 proteins were obtained from immunoprecipitation using specific antibodies against PRDX2，9 of them were regulated by c-Myc gene. Conclusion：PRDX2 play roles in the development and metastasis of colorectal cancer. PRDX2 might be associated with c-Myc gene regulatory pathway.