HMGB1在过敏性紫癜患儿血清中的表达及其临床意义

Expression of high mobility group protein 1 in children with anaphylactoid purpura and its clinical significance

摘要:

目的：探讨高迁移率族蛋白1（high mobility group protein，HMGB1）在过敏性紫癜患儿血清中的表达及其临床意义。方法：分别利用ELISA法检测183例过敏性紫癜患儿发病期和康复期血清HMGB1表达量以及real-time PCR法检测HMGB1 mRNA相对表达。40例健康体检儿童作为对照组。结果：患儿发病期血清HMGB1蛋白浓度高于对照组（t=7.43，P=0.000）；患儿康复期血清HMGB1蛋白较发病期降低（t=34.257，P=0.000）。患儿发病期外周血HMGB1 mRNA相对表达量升高（t=10.362，P=0.000）；康复期较发病期明显降低（t=4.651，P=0.000）。患儿HMGB1蛋白在不同性别（t=0.032，P=0.974）、年龄（F=0.088，P=0.916）以及初发、复发（t=0.449，P=0.654）患儿血清中的浓度差异无统计学意义；在有无肾损伤（t=5.672，P=0.000）、有无关节症状（t=3.048，P=0.003）、有无消化道症状（t=2.050，P=0.042）患儿血清中的浓度有明显差异。有肾损伤、有关节炎症状、有消化道症状患儿外周血HMGB1 mRNA相对表达分别高于无肾损伤（t=2.867，P=0.005）、无关节炎症状（t=2.733，P=0.007）、无消化道症状（t=2.366，P=0.019）患儿。结论：HMGB1作为一种新的生物学指标，有助于诊断过敏性紫癜活动期及其并发症，有利于临床治疗选择。

Abstract:

Objective：To investigate the expression of high mobility group protein 1（HMGB1） in children with anaphylactoid purpura and its clinical significance. Methods：Expression of serum HMGB1 in 183 children with anaphylactoid purpura was detected by ELISA. HMGB1 mRNA relative expression was detected by real-time PCR. Forty healthy children were enrolled as control group. Results：Serum HMGB1 concentration was higher in anaphylactoid purpura group than in control group（t=7.43，P=0.000） during disease period and after recovery（t=34.257，P=0.000）. HMGB1 mRNA in peripheral blood increased（t=10.362，P=0.000），and decreased after recovery（t=4.651，P=0.000）. There were differences in HMGB1 protein concentration between different genders（t= 0.032，P=0.974），among different ages（F=0.088，P=0.916） as well as between initial onset and recurrence（t=0.449，P=0.654）. There were significant differences in HMGB1 protein concentration between those with and without kidney injury（t=5.672，P=0.000），joint symptoms（t=3.048，P=0.003），and gastrointestinal symptoms（t=2.050，P=0.042）. HMGB1 mRNA relative expression in peripheral blood was higher in those with kidney damage，arthritis symptoms，gastrointestinal symptoms than in those without kidney injury（t=2.867，P=0.005），arthritis symptoms（t=2.733，P=0.007） and gastrointestinal symptoms（t=2.366，P=0.019）. Conclusion：As a new biological marker，HMGB1 could play an important role in diagnosis and treatment of complications of anaphylactoid purpura.