有序排列聚甲基丙烯酸甲酯电纺纳米纤维作为大鼠原代雪旺细胞负载支架的可行性研究

Feasibility of taking aligned electrospun polymethylmethacrylate nanofibers as rat primary Schwann cell loading scaffold

摘要:

目的：探讨有序聚甲基丙烯酸甲酯（polymethylmethacrylate，PMMA）电纺纳米纤维作为雪旺细胞负载支架的潜力。方法：构建有序PMMA电纺纳米纤维，以随机PMMA电纺纤维作为对照，纯化大鼠原代雪旺细胞并在纤维结构上进行培养，利用慢病毒技术转染绿色荧光蛋白基因作为显色手段，观察有序PMMA电纺纤维的拓扑线索在定向引导SCs生长上的作用，分析细胞对纤维结构的依从性；并在此基础上，持续动态观察雪旺细胞对有序电纺纤维的依从性，从而评价有序PMMA电纺纳米纤维作为神经损伤后植入性雪旺细胞负载支架的潜力。结果：雪旺细胞在随机及有序PMMA电纺纤维上均能顺利贴壁并生长；较之随机电纺纤维，雪旺细胞对有序纤维具有更好的依从性，能够受其接触引导形成和纤维走行方向一致的定向生长，并能够生成更长的细胞突起（P=0.0079）；动态的观察则进而显示SCs对有序电纺纳米纤维的拓扑线索能维持稳定的依从性。结论：有序PMMA电纺纳米纤维具有作为神经损伤后植入性雪旺细胞负载支架的潜力。

Abstract:

Objective：To evaluate the potentiality of taking aligned polymethylmethacrylate（PMMA） electrospun nanofibers as Schwann cell loading scaffold. Methods：Aligned electrospun nanofibers were fabricated and primary rat Schwann cells were cultured on them；random PMMA nanofibers were served as control. Green fluorescent protein gene was tranfected by lentivirus and made as the report－ing gene；the contact guidance exerted by the topographic feature of nanofibers on the growth of Schwann cells was revealed，and the microcosmic relation between the cell and the fiber was showed. Based on the findings mentioned above，the dynamic compliance of Schwann cells to aligned PMMA nanofibers was exhibited. Furthermore，potentiality of taking PMMA electrospun nanofibers as Schwann cell loading scaffold after neural injury was evaluated. Results：Schwann cell could adhere and grow both on random and aligned PMMA nanofibers. Compared with random nanofibers，aligned nanofibers could provide more supporting to Schwann cell，and on aligned fibers Schwann cells could grow along the axis of the fibers and form longer cell process（P=0.0079）. Dynamic observation showed good com－pliance of Schwann cells to the topographic features of aligned nanofibers. Conclusion：Aligned PMMA electrospun nanofibers have the potentiality of being transplantable scaffold for loading Schwann cells after neural injury.