Th17细胞及相关因子在多发性硬化中表达变化及临床意义

Changes in expression of Th17 cells and related cytokines in multiple sclerosis and its clinical significance

摘要:

目的：探讨多发性硬化（multiple sclerosis，MS）患者体内Th17细胞及相关细胞因子表达的变化及临床意义。方法：收集MS患者27例（MS组）和健康对照20例（对照组）。采用逆转录聚合酶链反应（reverse transcription-polymerase chain reaction，RT-PCR）检测外周血单个核细胞（peripheral blood mononuclear cells，PBMCs）中白介素（interleukin，IL）-17 mRNA的表达；采用酶联免疫法（enzyme linked immunosorbent assay，ELISA）检测IL-17、IL-23和IL-6水平；采用流式细胞仪检测Th17细胞的表达。结果：MS组外周血PBMCs中IL-17 mRNA的表达明显高于对照组（P<0.05）；MS组外周血中Th17细胞及相关细胞因子IL-17、IL-23和IL-6的表达也明显高于对照组（P<0.05）。结论：Th17细胞及其相关细胞因子可能参与了MS的发生与进展，检测Th17细胞对诊断MS具有一定的临床意义。

Abstract:

Objective：To investigate the expression of change of Th17 cells and related cytokines in multiple sclerosis patients and its clinical significance. Methods：Totally 27 multiple sclerosis patients（MS group） and 20 healthy subjects（control group） were selected. RT-PCR was used to detect IL-17 mRNA；ELISA was used to detect IL-17，IL-23 and IL-6；FACS was used to detect Th17 cells in peripheral blood. Results：Level of IL-17 mRNA was significantly increased in MS group（P<0.05）. When compared with that control group，expression of Th17 cells and related cytokines（IL-17，IL-23 and IL-6） was significantly increased in MS group（P<0.05）. Conclusion：Th17 cells and related cytokines may participate in the pathogenesis of multiple sclerosis. Detection of Th17 cells could have clinical value in diagnosis of multiple sclerosis.