按摩促进骨骼肌损伤后肌纤维再生机理的研究

Mechanism of using massage to promote the fiber formation in skeletal muscle damage

摘要:

目的：研究按摩对兔股四头肌损伤成肌调节因子 Myf5 mRNA和 MyoD mRNA的影响，探讨按摩促进肌纤维再生的作用机理。方法：将健康成年新西兰大白兔 42 只，体质量（2.0±0.5） kg，随机分为4组，正常组（A组，n=3）、损伤组（B组，n=3）、按摩组（C组，n=18）和自然恢复组（D组，n=18）。A组实验动物不作任何处理，作为正常对照；用重物打击法将B、C、D 3组实验兔左后肢股四头肌进行损伤造模。C组于造模后第4天开始按摩，按摩器转速2 600 r/min，按摩时间15 min，每天1次；D组不予按摩。C、D组于损伤第5天及第10天各取9只实验动物取股四头肌样本，进行HE染色观察组织的病理变化，并采用real-time PCR检测各组 Myf5 mRNA和 MyoD mRNA的相对表达量，与A、B 2组股四头肌标本进行比较。结果：成肌调节因子Myf5 mRNA的表达，A、B组分别为（7.82±0.19）、（4.99±0.17），损伤第5天C、D组分别为（5.28±0.15）、（5.78±0.04），损伤第10天C、D组分别为（5.95±0.16）、（6.26±0.26），C、D组相比，差异有统计学意义（P<0.01）；MyoD mRNA的表达，A、B组分别为（14.94±0.12）、（12.12±0.03），损伤第5天C、D组分别为（12.52±0.12）、（12.88±0.14），损伤第10天C、D组分别为（13.28 ±0.02）、（13.81±0.17），C、D组相比，差异有统计学意义（P<0.01）；HE染色显示，C组充血、水肿减轻，与D组相比，肌纤维排列较整齐，直径增粗。结论：按摩能够促进兔损伤股四头肌肌胶原纤维合成，利于损伤组织的修复，其机理可能是通过促进成肌调节因子Myf5 mRNA和MyoD mRNA的表达有关。

Abstract:

Objective：To investigate the mechanism of using massage to promote muscle fiber regeneration by studying on the effect of massage on myogenic regulatory factor of rabbit quadriceps injury Myf5 mRNA and MyoD mRNA. Methods：The 42 healthy adult New Zealand white rabbits，weighting（2.0±0.5） kg，were randomly divided into four groups：normal group（A，n=3），control group before massage（B group，n=3），massage group（C group，n=18） and natural recovery group（D group，n=18）. No treatment was used in A group while hitting by a homemade device was used in B，C and D groups to build rabbits’ quadriceps injury model in right and hind limb. In group C，massage was provided in the first four days after modeling，with speed of 2 600 r/min and frequency of 1/d，for 15 min. In group D，no massage was given. Quadriceps specimens of nine rabbits in C group and D group were got respectively on the 5th d and 10th d after modeling and pathological changes were observed by HE staining. The relative expression of Myf5 mRNA and MyoD mRNA was detected by real-time quantitative PCR. Results：The myogenic regulatory factor Myf5 mRNA expression was （7.82 ±0.19） and （4.99±0.17） in A and B groups，（5.28±0.15） and （5.78 ± 0.04） in C and D groups on the 5th d after modeling，（5.95 ± 0.16） and （6.26±0.26） in C and D groups on the 10th d after modeling，with statistical differences（P<0.01）. MyoD mRNA expression was （14.94±0.12） and （12.12±0.03） in A and B groups，（12.52±0.12） and （12.88±0.14） in C and D groups on the 5th d after modeling，（13.28 ± 0.02） and （13.81±0.17） in C and D groups on the 10th d after modeling，with statistical differences（P<0.01）. HE staining showed that the damage was mitigated and the muscle fibers were more orderly with larger diameter in C groups than in D groups. Conclusion：Massage can promote the synthesis of collagen fibers of injured quadriceps muscles in rabbits，which will help re－pair damaged tissues；its mechanism may be through the pro－motion of myogenic regulatory factor Myf5 mRNA and MyoD mRNA expression.