Mimics与Micro-CT内置软件测量小鼠颅骨缺损骨再生的对比研究

Evaluation on bone regeneration in mouse calvarial defect model using Mimics vs. Micro-CT scanner’s built-in software

摘要:

目的：对比分析Mimics软件与显微-CT（Micro-computed tomography，Micro-CT）内置分析软件对颅骨缺损内新生骨量及骨愈合率的测量结果，评价2种测量方法的差异性。方法：在小鼠右侧颅顶骨制备直径4 mm圆形骨缺损，分别于术后当天、6周、12周，对小鼠颅骨进行活体Micro-CT扫描，然后利用Micro-CT扫描仪内置软件重建颅骨缺损的三维图像并测量缺损内新生骨体积及愈合率，且利用Mimics软件对Micro-CT扫描的二维图片进行三维重建，并测量缺损内新生骨体积及愈合率。结果：与术后6周相比，术后12周Micro-CT扫描仪内置软件和Mimics软件测量的缺损内新生骨体积和骨愈合率均增加，差异有统计学意义（P<0.05）；术后6周和12周，Mimics软件的测量结果与Micro-CT扫描仪内置软件测量的颅骨缺损内新骨体积及骨愈合率相比，差异无统计学意义（P >0.05）。结论：缺损术后6周和术后12周，Mimics软件对Micro-CT扫描后的二维图片进行重建并计算出的颅骨缺损新生骨体积及骨愈合率，与Micro-CT扫描仪内置软件的测量结果均一致，且测量方法更简单。

Abstract:

Objective：To compare the results of bone regeneration condition in mouse calvarial defects measured by Mimics or Micro-CT scanner’s built-in software and to evaluate the differences of the two methods. Methods：Defect of 4 mm in diameter was prepared on the right cranium of mice，and in vivo Micro-CT scan was performed immediately after surgery，6 weeks and 12 weeks postopera－tively. Then new bone volume and bone healing rate within the defect were measured after reconstructing 3D images by Micro-CT scanner’s built-in software. The new bone volume and bone healing rate within the defect were quantitatively analyzed using Mimics software. The 2D data of Micro-CT were imported into Mimics software and 3D images were reconstructed by it as well. Results：The bone regeneration volume and bone healing rate at 12 weeks postoperatively were significantly higher than those at 6 weeks postoper－atively，no matter measured by Micro-CT scanner’s built-in software or Mimics software（P<0.05）. In addition，there was no signifi－cant difference in the new bone volume and bone healing rate between the results of Mimics software and the results of Micro-CT scanner’s built-in software at 6 weeks and 12 weeks postoperatively（P<0.05）. Conclusion：Similar results of new bone regeneration within the calvarial defect are obtained by the two softwares at 6 weeks and 12 weeks postoperatively . However，mimics software is simpler to operate than Micro-CT scanner’s built-in software.