TRIM28在非小细胞肺癌中的表达及其对肺腺癌PAa细胞生长的影响

Expression of TRIM28 in non-small cell lung cancer and its effect on the growth of lung adenocarcinoma PAa cell

摘要:

目的：鉴定TRIM28基因在非小细胞肺癌（non-small cell lung cancer，NSCLC）中的表达；探讨TRIM28基因对NSCLC生长的影响。方法：免疫组织化学技术检测TRIM28在NSCLC组织中的表达。通过RNA干扰技术抑制肺腺癌PAa细胞系中TRIM28基因表达后，克隆形成实验和MTT实验观察细胞的生长和增殖情况；流式细胞仪分析细胞周期改变。结果：在48例NSCLC组织中，24例（50.0%）阳性表达TRIM28，而在癌旁正常肺组织中均不见TRIM28阳性染色（ ?字2=27.034，P=0.000）。TRIM28基因被干扰后，肺腺癌PAa细胞的活性和生长明显受到了抑制，并且引起细胞周期G1/S期阻滞。结论：TRIM28基因和蛋白在NSCLC组织中表达上调；siRNA阻断TRIM28表达后抑制肺腺癌PAa细胞生长，提示TRIM28可能作为新的NSCLC治疗靶点。

Abstract:

Objective：To identify the expression of TRIM28 in non-small cell lung cancer（NSCLC），to study the effect of TRIM28 gene on the growth of non-small cell lung cancer. Methods：Immunohistochemistry was used to detect the expression of TRIM28 in clinical tissue of non-small cell lung cancer patients. After TRIM28 gene was inhibited by RNA interference assay，soft agar assay and MTT cell proliferation assay were used to observe the growth and proliferation of lung adenocarcinoma PAa，and flow cytometry was used to an－alyze the cell cycle. Results：TRIM28 positive staining was observed in 24 of 48（50.0%）non-small cell lung cancer tissue samples，whereas no staining was observed in any of the normal portions of the same tissues（ ?字2=27.034，P=0.000）. TRIM28 knockdown resulted in a decrease in cell proliferation and a significant population in G1/S fraction. Conclusion：TRIM28 expresses highly in non-small cell lung cancer tissues. The growth of lung adenocarcinoma PAa transfected with TRIM28 siRNA is inhibited suggesting that TRIM28 may be new therapy target for NSCLC.