白细胞介素28B基因多态性以及表达水平对慢性丙型肝炎患者抗病毒治疗效果的影响研究

Effect of IL-28B gene polymorphism and expression on antiviral therapy of patients with chronic hepatitis C

摘要:

目的：探索白细胞介素28B（interleukin-28B，IL-28B）在慢性丙型肝炎（chronic hepatitis C，CHC）患者抗病毒治疗中的作用。方法：收集到46例CHC患者的抗凝全血，采用多重高温连接酶检测反应检测IL-28B单核甘酸多态性位点rs8099917。同时收集12例健康者血清，46例CHC患者初治时的血清及治疗后12周时其中44例CHC患者的血清，采用ELISA法检测IL-28B水平，并与肝功能进行相关分析。结果：①在46例CHC患者中，rs8099917 TT基因型约占87.0%（40/46），rs8099917 GT基因型约占13.0%（6/46）。血清IL-28B水平在CHC组与健康组，基因1b型与非1b型CHC患者组，高病毒载量与低病毒载量组及rs8099917 TT基因型组与GT基因型组间差别无统计学意义（P均>0.05），与丙氨酸氨基转移酶、门冬氨酸氨基转移酶、谷氨酰转肽酶、血小板之间无相关性。②血清IL-28B在44例CHC治疗后12周达到早期病毒学应答（early virological response，EVR）组比未达到EVR组高，差别有统计学意义（P=0.000）。结论：重庆地区的CHC患者rs8099917基因型以TT型为主。血清IL-28B在rs8099917 TT基因型组与GT基因型组表达虽无差别，但对CHC患者的EVR有重要影响。

Abstract:

Objective：To explore the role of interleukin-28B during antiviral treatment in patients with chronic hepatitis C. Methods：Anticoagulant whole blood of 46 patients with chronic hepatitis C were collected.Interleukin-28B single nucleotide polymorphism of rs8099917 genotype was tested by improved multiplex ligation detection reaction.At the same time，102 cases of serum samples were collected，including 46 cases of chronic hepatitis C before treatment and 44 cases of chronic hepatitis C after 12-week therapy，12 cases of healthy controls.The serum levels of interleukin-28B were examined by ELISA and the correlation of interleukin-28B with liver function was analyzed. Results：（1）rs8099917 TT genotype was 87.0%（40/46） and rs8099917 GT genotype was 13.0%（6/46） a－mong the 46 cases of chronic hepatitis C. There was no statistical significance in the serum levels of interleukin-28B between chronic hepatitis C group and healthy group，hepatitis C viral genotype 1b group and non-1b group，high viral load group and low viral load group，rs8099917 TT genotype group and GT genotype group（P>0.05）. There was no correlation between serum interleukin-28B and aspartate aminotransferase，alanine aminotransferase，γ-glutamyl transferase，platelet.（2）There were significant differences in expres－sion of interleukin-28B among 44 patients with chronic hepatitis C after 12-week therapy，which was higher in the early virological response group than in non early virological response group（P=0.000）. Conclusion：The rs8099917 genotypes of patients with chronic hepatitis C are mainly composed by TT genotype in Chongqing area.There is no significant difference in the levels of interleukin-28B between rs8099917 TT genotype group and rs8099917 GT genotype group，but the expression of interleukin-28B have ef－fect on early virological response.