姜黄素联合FOLFOX方案对胃癌细胞株BGC-823的抑制和诱导凋亡的作用

Effect of curcumin combined FOLFOX regimen on proliferation inhibition and apoptosis-inducing of gastric carcinoma BGC-823

摘要:

目的：探讨姜黄素联合FOLFOX方案（奥沙利铂/氟尿嘧啶方案）对胃癌细胞株BGC-823的增殖和凋亡作用以及可能发生的机制。方法：以胃癌细胞株BGC-823 cell为研究对象，设置空白对照组、姜黄素组（10 μmol/L）、FOLFOX组[5-FU （0.1 mmol/L）+奥沙利铂（5 mmol/L）]和姜黄素联合FOLFOX组4组，采用CCK-8法测定细胞增殖抑制率，Hoechst染色观察细胞凋亡情况，real-time PCR和Western blot分别检测Bcl-2、Bax mRNA和蛋白表达变化，Caspase-3活性检测试剂盒测定Caspase-3活性。结果：①CCK8显示，与空白对照组比较，各用药组均抑制BGC-823细胞的增殖，姜黄素联合FOLFOX组的抑制作用最明显，差异均有统计学意义（P<0.05）；②Hoechst染色结果显示，各用药组凋亡细胞明显多于空白对照组，姜黄素联合FOLFOX组凋亡细胞最明显；③real-time PCR和 Western blot证实，与空白对照组比较，各用药组Bcl-2 mRNA、Bcl-2蛋白降低而Bax mRNA、Bax蛋白升高，姜黄素联合FOLFOX组变化更明显，差异均有统计学意义（P<0.05）；④Caspase-3活性检测证实，各用药组Caspase-3活性均升高，姜黄素联合FOLFOX组最高，差异均有统计学意义（P<0.05）。结论：姜黄素联合FOLFOX能抑制胃癌细胞株BGC-823细胞增殖，诱导凋亡，机制可能与Bax激活/Bcl-2失活，活化Caspase-3信号通路有关。

Abstract:

Objective：To investigate the effect of curcumin combined folinic acid fluorouracil oxaliplatin（FOLFOX） on proliferation inhibition and apoptosis-inducing of the gastric cell line BGC-823，and to explore its possible mechanisms. Methods：Gastric cancer cell line BGC-823 cells were divided into blank control group，curcumin group（10 μmol/L），FOLFOX group（5-FU 0.1 mmol/L+Oxaliplatin 5 mmol/L）] and curcumin combined FOLFOX group. CCK-8 was used to detect the cell proliferation. Cell apoptosis was observed by Hoechst staining. The mRNA and protein expressions of Bcl-2 and Bax were measured by real-time quantitative PCR and Western blot. Caspase-3 vitality was tested by Caspase-3 test kit. Results：The proliferation inhibition rate of BCG-823 cell in curcumin combined FOLFOX group was significantly higher than those in other groups（P<0.05）. Hoechst staining showed that apoptotic cells in medicated groups were higher than those in control group，being the most obvious in FOLFOX group. Compared with those in the control group，the expressions of Bcl-2 mRNA，Bcl-2 protein were decreased in other groups，while the expressions of Bax mRNA，Bax proteins were increased with statistically significant differences（P<0.05）. Caspase-3 activity was also increased significantly in the medicated groups，especially in curcumin combined FOLFOX group（P<0.05）. Conclusion：Curcumin combined FOLFOX could inhibit the proliferation and induce apoptosis of GC-823 cells，which was related with the activation Bax / inactivation Bcl-2 and the activation of Caspase-3.