NRP2及miR-486-5p在胃癌中的表达及临床意义

Expression and clinicopathogical significance of NRP2 and miR-486-5p in gastric carcinoma

摘要:

目的：检测神经纤毛蛋白-2（neuropilin-2，NRP2）和miR-486-5p在胃癌组织中的表达，探讨其与胃癌淋巴结转移的关系。方法：收集65例胃癌组织及其配对癌旁正常粘膜组织，免疫组织化学法检测组织中NRP2的表达，real-time PCR检测miRNA-486-5p的表达，免疫组织化学法检测D2-40标记的微淋巴管密度（microlymphatic density，MLD），统计分析NRP2、miR-486-5p的表达与MLD相关性。结果：免疫组化提示NRP2在肿瘤中表达增高（78.5% vs. 29.2%， χ2=16.046，P=0.000），且与淋巴结转移密切相关（χ2=7.222，P=0.007）。real-time PCR结果显示肿瘤组织miR-486-5p表达明显下调（0.39±0.16 vs. 1.00±0.06，P=0.000），NRP2的表达水平与MLD呈正相关（r=0.384，P=0.015），miR-486-5p的表达水平与MLD呈负相关（r=-0.755，P=0.000）。结论：NRP2和miR-486-5p与胃癌发生发展密切相关，有望成为胃癌治疗的新靶点。

Abstract:

Objective：To explore the relationship between the expression of neuropilin-2（NRP2） and microRNA-486-5p（miR-486-5p） in gastric carcinoma（GC） and adjacent non-tumorous tissues and lymph node metastasis of GC. Methods：Sixty-five gastric carcinoma and adjacent non-tumorous specimens were collected. Immunohistochemistry（IHC） was used to detect NRP2 protein expression in tissues；quantitative real time PCR was used to detect the expression of miR-486-5p in tissues；microlymphatic density（MLD）was evaluated with lymphatic endothelium-specific marker-D2-40. The relationship between NRP2，miR-486-5p and MLD was analyzed. Results：Compared with those in normal gastric mucosa tissues，the expression of NRP2 was significantly up-regulated in GC tissues（P=0.000），while the expression of miR-486-5p was down-regulated significantly（P=0.000）. Statistic analysis revealed the expression of miR-486-5p in GC tissues was negatively correlated with MLD（P<0.001） and NRP2 was positive correlated with MLD（P=0.015）. Conclusion：The expression of NRP2 and miR-486-5p is closely associated with lymph node metastasis of gastric carcinoma，indicating that miR-486-5p and NRP2 might be used as targets for molecular therapy of gastric carcinoma.