HIF-1α和miR-210在宫颈鳞癌中的表达及其相关性

Expression of HIF-1α and miR-210 in cervical squamous cell carcinoma and their relationship

摘要:

目的：探讨宫颈癌组织中缺氧诱导因子-1α（hypoxia induced factor-1α）和MicroRNA 210（miR-210）之间的关系，为宫颈癌的早期发现寻找参考指标。方法：采用免疫组化En Vision法和real-time PCR分别检测52例宫颈鳞癌及20例正常宫颈上皮组织中 HIF-1α和 miR-210的表达情况，用Spearman秩相关检验分析癌组织中HIF-1α和miR-210之间的相关性。结果：宫颈鳞癌组织细胞大部分胞核表达HIF-1α，少部分胞核和胞浆同时表达，HIF-1α在宫颈鳞癌组织中的表达率为73.1%（38/52），正常宫颈组织细胞中无表达。miR-210在宫颈癌组织中的表达水平较正常宫颈组织显著上调，正常宫颈组织miR-210的表达水平为1.16±0.76，宫颈鳞癌组织中其表达水平为5.04±1.28（P=0.000）；在52例宫颈癌组织中，HIF-1α蛋白表达水平与miR-210表达呈正相关（r=0.43，P=0.001）。HIF-1α蛋白在正常宫颈组织中均无阳性表达，与miR-210无相关性。在所有72例样本中进行相关性分析，发现两者的表达呈正相关（r=0.67，P=0.000）。说明随着HIF-1α蛋白表达的增加，miR-210则随着增加。结论：HIF-1α和miR-210在宫颈鳞癌组织中均呈高表达，且两者有一定的相关性。推测HIF-1α和 miR-210与宫颈癌的发生发展相关，有助于进一步揭示宫颈癌的发病机制。

Abstract:

Objective：To explore the correlation between hypoxia induced factor-1α（HIF-1α） and MicroRNA210（miR-210） in cer－vical cancer． Methods：The expression of HIF-1α protein was detected by immunohistochemistry. Poly（A）-RT-qPCR was used to de－tect the expression of miR-210 in cervical cancer and normal cervical tissues. The correlation between the expression of miR-210 and HIF-1α was analyzed in cervical cancer. Results：The expression of HIF-1α in cervical cancer was 73.1% （38/52），but HIF-1α was not detected in normal cervical tissues；the expression of miR-210 was significantly increased in cervical cancer tissues than in normal cervical tissues（P=0.000）. The expression of miR-210 in normal cervical tissues and cervical cancer was 1.16±0.76 and 5.04±1.28，respectively. The expression of HIF-1α and miR-210 were positively correlated in 52 cases of cervical cancer（r=0.43，P=0.001）. The expressions of HIF-1α and miR-210 were not correlated in normal cervical tissues. The expressions of HIF-1α and miR-210 were positively correlated in 72 cases（r=0.67，P=0.000）. Conclusion：The expression of HIF-1α and miR-210 are highly expressed in SCC tissues. HIF-1α and miR-210 are positively correlated in cervical cancer. It is predicted that HIF-1α and miR-210 are re－lated with the occurrence and development of cervical cancers，which is conductive to revealing the pathogenesis of cervical cancer.