口腔扁平苔藓患者外周血Th1/Th2型细胞因子表达及意义

Expression and significance of Th1/Th2 cytokines in peripheral blood of patients with oral lichen planus

摘要:

目的：了解口腔扁平苔藓（oral lichen planus，OLP）患者外周血中 Th1、Th2 型细胞因子表达水平，探讨 Th1/Th2 平衡在 OLP 发病中的作用及意义。方法：采用流式细胞术（cytometric bead array，CBA）检测 51例 OLP 患者（网纹型 23 例，充血糜烂型 28 例）Th1 型细胞因子（TNF-α、 IFN-γ、IL-2）及 Th2 型细胞因子（IL-4、IL-6、IL-10）表达水平，并分析其与 OLP 临床特征相关性。结果：①OLP 患者血清中 Th2 型细胞因子的IL-6 与 IL-10 的表达水平明显高于正常对照组（P＜0.05），但 Th1 型细胞因子 TNF-α、IFN-γ、IL-2 及 Th2 型细胞因子 IL-4 表达水平与正常组差异无统计学意义（P ＞0.05）；②Th2 型细胞因子 IL-6 的表达水平与 OLP 临床类型、临床体征、临床症状均成正相关，IL-10 的表达水平与 OLP 临床类型、临床体征均成正相关（相关系数0.285～0.364；P＜0.05）。结论：OLP 患者外周血中 Th1 型细胞因子的表达水平与正常对照组Th1 型细胞因子表达水平差异无明显改变，而Th2 型细胞因子升高，表现为 Th2 占优势的免疫应答；Th2 型细胞因子 IL-6、IL-10 表达与 OLP 患者病情严重程度呈正相关，对OLP 免疫治疗有一定指导意义。

Abstract:

Objective：To investigate the expression of Th1/Th2 cytokines in peripheral blood of patients with oral lichen planus（OLP） and to discuss the role and significance of Th1/Th2 cytokines in the pathogenesis of OLP. Methods：Totally 51 cases of OLP with dif－ferent clinical characters（including 23 cases of reticular OLP and 28 cases of hyperaemia erosion OLP） were enrolled. Cytometric bead array was employed to detect the level of type 1 cytokines（TNF-α，INF-γ，IL-2） and type 2 cytokines（IL-4，IL-6，IL-10） of T helper cells in serum of patients with OLP. The relationship between the levels of these cytokines and the clinical characteristics of patients with OLP was analyzed. Results：Expression levels of IL-6 and IL-10 in the peripheral blood of patients with OLP were sta－tistically increased compared with those of controls（P＜0.05），but no statistical difference was observed in level of Th1 cytokines（TNF-α，IFN-γ，IL-2） and IL-4 between OLP patients and controls（P＞0.05）. Expression levels of IL-6 and IL-10 were positively correlated with clinical characters（r：0.285-0.364；P＜0.05）. Conclusion：Th1 cytokines remain unchanged and Th2 cytokines increase in OLP patients，showing the advantage of Th2 immune response. Levels of IL-6 and IL-10 are positively correlated with disease severity，which may be helpful for OLP immunotherapy.