肝癌组织中FN和PTEN的表达及其临床意义

Expression of FN and PTEN in hepatocellular carcinoma and its clinical significance

摘要:

目的：探讨抑癌基因纤黏连蛋白（fibronectin，FN）、张力蛋白同源基因蛋白（phosphatase and tensin homology deleted on chromosome ten，PTEN）在肝细胞性肝癌（hepatocellular carcinoma，HCC）组织中的表达及其临床意义。方法：收集215例HCC、癌旁组织和19例正常肝组织，用RT-PCR、Western blot、免疫组化SP法检测FN、PTEN mRNA和蛋白表达，分析FN和PTEN表达与HCC临床病理特征的关系。结果：肝癌组织中FN mRNA和蛋白表达均明显高于癌旁组织及正常肝组织（F=142.334，P=0.000）；而PTEN低于癌旁组织及正常肝组织，且在癌旁组织中的表达亦明显低于正常肝组织（F=80.861，P=0.000）。FN表达阳性患者组较阴性患者组生存时间短；而PTEN表达阳性患者较阴性患者生存时间长，且在1年以后两者生存期有统计学意义（P<0.05）。结论：HCC癌组织中 FN、PTEN在肝癌组织中存在异常表达，两者异常表达可能在肝癌的发生发展、侵袭转移中起一定的促进或抑制作用。

Abstract:

Objective：To investigate the expressions of fibronectin（FN） and phosphatase and tensin homology deleted on chromosome ten（PTEN） in hepatocellular carcinoma（HCC） tissue and its clinical significance. Methods：Totally 215 cases of HCC（paraneoplastic tissues） and 19 cases of normal liver tissues were collected. The expressions of FN and PTEN mRNA and protein were detected by RT-PCR，Western blot and immunohistochemical SP method respectively. The correlation between the expressions of FN and PTEN and clinicopathologic features of HCC was analyzed. Results：The expressions of FN mRNA and protein in hepatocellular carcinoma tissue were significantly higher than those in paraneoplastic and normal liver tissue. The expressions of PTEN were significantly lower than those in paraneoplastic and normal liver tissue（F=142.334，P=0.000）. And the expression in paraneoplastic tissues was obviously lower than that in normal liver tissue（F=80.861，P=0.000）. The survival time of FN positive expression group was shorter than that of negative groups；the survival time of PTEN positive expression group was longer than that of negative group；both had statistical sig－nificances after one year（P<0.05）. Conclusion：FN and PTEN ae abnormally expressed in HCC tissue，which might play a promoting or inhibiting effect in the occurrence，development，invasion and metastasis of HCC.