改良酚-氯仿法提取及不同试剂盒扩增检测陈旧性人骨DNA

Modified phenol-chloroform method and amplification with different kits in human obsolete bone

摘要:

目的：探讨改良酚-氯仿法提取及不同试剂盒扩增对陈旧性人骨DNA检测的效果。方法：用传统和改良的酚-氯仿法对30例陈旧性人骨进行DNA提取，用Identifiler-plus试剂盒、Minifiler试剂盒和GodeneyeTM 20A试剂盒行荧光标记和复合扩增，Genetic Analyzer软件对基因位点进行检测和分析。结果：改良酚-氯仿法提取及3种试剂盒对陈旧性人骨DNA基因位点检出469、262、588个，高于传统法的307、175、436个。结论：改良酚-氯仿法、3种试剂盒PCR复合扩增能对陈旧性人骨DNA进行有效提取和检测。

Abstract:

Objective：To explore the detection results of DNA in human obsolete bone extracted by modified phenol-chloroform method and amplified with different kits. Methods：DNA in 30 femoral bones were extracted by modified phenol-chloroform method，fluorescent label and multiplex amplification with identifiler-plus kit，minifiler kit and godeneyeTM 20A kit，while the gene loci was detected and analyzed by genetic analyzer software. Results：The DNA gene loci in in human oboslete bone were 469，262，588 re－spectively，which were higher than those of 307，175，436 by traditional method. Conclusion：DNA in human obsolete bone might be extracted and detected effectively by modified phenol chloroform method and multiplex amplification with 3 kits.