博尔纳病病毒通过磷蛋白调节miR-134影响人少突胶质瘤细胞的增殖研究

Borna disease virus encoded phosphoprotein down-regulates the proliferation of human oligodendrocytes by regulating miR-134

摘要:

目的：探讨博尔纳病病毒（Borna disease virus，BDV）在感染宿主细胞中，miR-134对人少突胶质瘤细胞（oligodendro－cyte，OL）增殖水平的影响。方法：应用real-time PCR方法检测正常的OL细胞和BDV感染的OL细胞（OL/BDV）中的miR-134表达水平，同时分别将BDV phosphoprotein（P24），nucleoprotein（P40）蛋白真核表达载体转染OL细胞，检测对miR-134的作用；通过miR-134过表达和BDV感染OL细胞，采用流式细胞仪检测miR-134和BDV对OL细胞增殖的影响。结果：real-time PCR表明miR-134在OL、OL/BDV 细胞中的表达出现明显差异（P=0.000），并且BDV 中的P24蛋白对miR-134有明显的上调作用（P=0.000），但P40对miR-134没有明显作用（P=0.139）；流式细胞仪结果显示miR-134过表达（P=0.016）和BDV感染（P=0.001）均可以抑制OL细胞的增殖能力。结论：BDV感染可通过P24蛋白上调OL细胞中miR-134的表达，进而导致细胞增殖水平下降。

Abstract:

Objective：To investigate the proliferation of oligodendrocyte（OL） cells induced by miR-134 in Borna disease virus（BDV） infection. Methods：The expression levels of miR-134 in OL and OL/BDV were detected by real-time PCR. BDV phosphoprotein （P24） or nucleoprotein（P40） gene expression vector was used to transfect OL cells respectively；the effects of BDV P24 and P40 pro－teins on the expression of miR-134 were detected by real-time PCR. The OL cells proliferation ability was investigated by flow cytom－etry（FCM），along with miR-134 transfection and BDV infection. Results：There were significant differences in expression of miR-134 between OL and OL/BDV cells by real-time RT-PCR（P=0.000）. The expression of miR-134 was significantly increased by BDV P24 overexpression（P=0.000），but not by BDV P40（P=0.139）. Both overexpression of miR-134（P=0.016） and BDV infection（P=0.001） down-regulated the proliferation of OL cells. Conclusion：BDV P24 protein down-regulates the proliferation of OL cells by up-regu－lating miR-134.