C6胶质瘤肝脏转移模型的建立及其磁共振检测

Application of 3.0T magnetic resonance imaging in motoring liver hematogenous metastasis model established by C6 cell injection

摘要:

目的：建立裸鼠胶质瘤肝脏转移模型，探讨3.0T磁共振成像（magnetic resonance imaging，MRI）对模型肝脏转移灶检测的可行性。方法：手术暴露裸鼠肝门静脉，经肝门静脉注射C6胶质瘤细胞至36只裸鼠肝脏，分别于注射胶质瘤后的7 d、14 d和21 d随机抽取建模裸鼠12只行3.0T MRI扫描，之后取建模裸鼠肝脏组织对其进行大体形态学观察、HE染色，比较3种方法对肿瘤转移灶的检出能力以及所测肿瘤结节大小的差异，并使用S100beta进行免疫组学染色。结果：MRI影像学对肿瘤转移灶的检出时间要晚于HE染色（Wald χ2=5.01，P=0.025），而较大体标本改变出现时间无差异（Wald χ2=0.03，P=0.853）；且14 d与21 d时MRI所测肿瘤结节直径与HE染色以及大体标本所见肿瘤结节直径之间亦有差异（F14 d=14.28，P=0.000；F21 d=1 123.23，P=0.000）。组织切片S100beta免疫组化结果证实肝脏肿瘤结节为C6胶质瘤。且肿瘤转移灶在MRI T2WI上呈高信号改变，对较大肿瘤结节3.0T MRI能显示其内细节及瘤周水肿。结论：该方法可成功构建胶质瘤血行肝脏转移模型；且3.0T MRI可用于该模型鼠肿瘤转移灶的检测。

Abstract:

Objective：To establish liver hematogenous metastasis model in nude mice through injection of C6 glioma cells to hepatic portal vein and to investigate the feasibility and accuracy of 3.0T magnetic resonance imaging（MRI） in detection of the liver metas－tasis model. Methods：Hepatic portal veins of 36 nude mice were exposed by surgical operation. Then cultured C6 glioma cells were injected into hepatic portal veins of the 36 nude mice. Every time，12 nude mice were randomly sampled for magnetic resonance scanning on 7th，14th，21st d. After scanning，these mice were sacrificed. All livers of these mice were collected for pathological exam－ination，the ability for detection of tumor metastasis and the diameter of metastatic nodules detected by the three methods were com－pared，and immunohistochemical staining was used to assay the expression of S100beta. Results：The time metastatic nodules could be detected by MRI was latter than that by HE staining（Wald χ2=5.01，P=0.025），with no obvious difference when comparing with the time morphological changes revealed（Wald χ2=0.03，P=0.853）. And there were significant differences among the diameter of metastatic nodules detected by the three methods on 14 th d and on 21st d（F14 d=14.28，P=0.000；F21 d=1 123.23，P=0.000）. Immunohistochemical staining of S100beta confirmed metastastic nodules were C6 glioma. Metastases manifested high signal in MRI T2WI characteristics. 3.0T MRI obtained more details about metastatic nodules and also showed edema around tumors. Conclusion：The liver metastasis model can be successfully established by portal vein injection of C6 cells. A clinical 3.0T MRI scanner can be used to detect formation and transform of liver metastases in this animal model.