白血病细胞中Beta-Arrestin1与EZH2分子结合研究

Analysis of the interaction of Beta-Arrestin1 and EZH2 in leukemia cells

摘要:

目的：检测急性淋巴细胞白血病细胞（acute lymphoblastic leukemia，ALL）CCRF-CEM和Raji细胞中Beta-抑制蛋白1（Beta-Arrestin1）与Zeste增强子同源物-2蛋白（enhancer of Zeste homolog 2，EZH2）是否存在结合。方法：白血病细胞中，Western blot检测Beta-Arrestin1与EZH2表达水平，激光共聚焦（Confocal）检测 Beta-Arrestin1与EZH2在细胞中的位置与共定位；免疫共沉淀（CO-IP）检测Beta-Arrestin1与EZH2结合能力。结果：Beta-Arrestin1与EZH2在白血病K562、CCRF-CEM和Raji细胞中均有表达。激光共聚焦结果显示Beta-Arrestin1与EZH2均在K562、CCRF-CEM和Raji细胞内共定位，CO-IP结果显示在K562，CCRF-CEM和Raji细胞中Beta-Arrestin1与EZH2结合。结论：在CCRF-CEM和Raji中，Beta-Arrestin1可与EZH2结合。

Abstract:

Objective：To test that if Beta-Arrestin1 could bind with enhancer of Zeste homolog 2（EZH2） in acute lymphoblastic leukemia（ALL） CCRF-CEM and Raji cells. Methods：The protein levels of Beta-Arrestin1 and EZH2 in the leukemia cells were determined by Western blot. The location of Beta-Arrestin1 and EZH2 in the leukemia cells was measured by confocal microscopy. Co-immunoprecipitation（CO-IP） was examined for the binding of Beta-Arrestin1 with EZH2 in leukemia cells. Results：Western blot results showed that b/Beta-Arrestin1 and EZH2 expressed in those three leukemia cells. Confocal data showed the colocaliza－tion of Beta-Arrestin1 and EZH2 in K562，CCRF-CEM and Raji cells. CO-IP assay illustrated that Beta-Arrestin1 bind with EZH2 in three leukemia cells. Conclusion：Beta-Arrestin1 could bind to EZH2 in ALL CCRF-CEM and Raji cells.