拘束应激对特应性皮炎样小鼠模型皮肤炎症影响的实验研究

Effect of restraint stress on the inflammation of atopic dermatitis－like animal model

摘要:

目的：观察拘束应激对特应性皮炎（atopic dermatitis，AD）样小鼠皮肤炎症的影响。方法：将28只6~8周龄雌性BALB/c小鼠分为4组：2，4-二硝基氯苯（2，4-dinitrochlorobenzene，DNCB）诱导-拘束应激组、DNCB诱导-对照组、丙酮诱导-拘束应激组和丙酮诱导-对照组，每组7只。第14天评估小鼠皮肤中炎症细胞浸润程度，Q-PCR方法检测皮损组织中干扰素-γ（interferon-γ，IFN-γ）、白细胞介素-4（interleukin-4，IL-4）和白细胞介素-13（interleukin-13，IL-13）等细胞因子的mRNA表达变化。结果：DNCB诱导-拘束应激组的真皮炎症细胞浸润数为42.710±3.944个，DNCB诱导-对照组的真皮炎症细胞浸润数为26.000±2.410个，有统计学差异（P<0.01）；相对于DNCB诱导-对照组，DNCB诱导-拘束应激组皮损组织中IL-4、IL-13等细胞因子的mRNA表达上升，差异有统计学意义（P<0.01）。结论：拘束应激加剧AD的炎症反应。

Abstract:

Objective：To study the effect of restraint stress on the inflammation changes of atopic dermatitis animal model. Methods： Twenty eight 6 to 8 weeks’ female BALB/c mice were divided into 4 groups：DNCB-induced model restraint stress group，DNCB-in－duced model restraint stress control group，acetone-induced model restraint stress group and acetone-induced model restraint stress control group. At the 14th day，mice were sacrificed and dermal infiltrated inflammatory cells and mRNA levels of inflammatory cy－tokines of skin lesions were analyzed by microscope histopathology and Q-PCR respectively. Results：In DNCB-induced model re－straint stress control group，the numbers of infiltrated inflammatory cells in dermal were significantly lower than DNCB-induced model restraint stress group（42.710±3.944） vs. （26.000±2.410）（P<0.01）. The mRNA levels of IL-4 and IL-13 were significantly elevated in DNCB-induced model restraint stress group. Conclusion：Restraint stress might accelerate the inflammatory response of atopic der－matitis.