NE、MMP-12在慢性阻塞性肺疾病发病机制中的作用

Role of neutrophil elastase and matrix metalloproteinase 12 in the pathogenesis of chronic obstructive pulmonary diseases

摘要:

目的：探讨中性粒细胞弹性蛋白酶（neutrophil elastase，NE）、基质金属蛋白酶12（matrix metalloproteinase 12，MMP-12）在慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）发病中的作用。方法：收集因肺部肿瘤行肺叶切除手术患者肺组织60例，其中非COPD者（A组）30例，COPD患者（B组）30例。患者术前均进行肺功能指标（FEV1％，FEV1/FVC％）检测；采用免疫组织化学方法检测NE、MMP-12在肺组织中的表达。结果：B组肺组织中NE、MMP-12的蛋白量表达较A组明显增强（P<0.05）；B组NE的蛋白表达量与FEV1％、FEV1/FVC％呈负相关（r分别为-0.474、-0.364，均P<0.05），B组MMP-12的蛋白表达量与FEV1％、FEV1/FVC％呈负相关（r分别为-0.409、-0.791，均P<0.05）。结论：NE、MMP-12在COPD患者肺组织中的表达上调并与肺功能指标相关，NE、MMP-12可能参与了COPD的气道炎症过程，也是引起气流阻塞的重要因素。

Abstract:

Objective：To explore the role of neutrophil elastase（NE） and matrix metalloproteinase 12（MMP-12） in the pathogenesis of patients with chronic obstructive pulmonary disease（COPD）. Methods：Surgically resected lung specimens from 60 patients with lung cancer were collected and divided into non COPD group（A group，30 cases） and COPD patients（B group，30 cases）. Pulmonary function indexes（FEV1%，FEV1/FVC%） were detected in all patients before operation. The expressions of NE and MMP-12 in lung tissue were detected by immunohistochemical method. Results：The protein expression of NE and MMP-12 in B group was signif－icantly higher than those in A group（P<0.05）. The expression of NE in B group was negatively correlated with FEV1%，FEV1/ FVC%（r=-0.474，-0.364；P<0.05，respectively），and the expression of MMP-12 in B group was also correlated with FEV1%，FEV1/FVC%（r=-0.409，-0.791，P<0.05，respectively）. Conclusion：The over-expression of MMP-12 and NE in lung tissue associated with lung function index. MMP-12 and NE may be involved in the airway inflammation，and play important role in the airway obstruction of COPD.